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Merck
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T6567

Sigma-Aldrich

胰蛋白酶 来源于猪胰腺

Proteomics Grade, BioReagent, Dimethylated

别名:

Porcine Trypsin, Trypsin for Mass Spectropetry

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About This Item

EC號碼:
232-650-8
MDL號碼:
MFCD01775455
分類程式碼代碼:
12352204
NACRES:
NA.56

生物源

Porcine pancreas

品質等級

200

產品線

BioReagent

溶解度

1 mM HCl: soluble 1 mg/mL, clear, colorless

運輸包裝

wet ice

儲存溫度

2-8°C

相关类别

一般說明

胰蛋白酶是一种丝氨酸蛋白酶，通常在生物化学和生物学中用作重要的酶试剂。这种方法产生了适用于蛋白质组学研究的高度纯化的胰蛋白酶产物。蛋白质组学等级非常适合用于溶液和凝胶胰蛋白酶消化。胰蛋白酶是一种丝氨酸蛋白酶，存在于几种脊椎动物的消化系统中。

應用

猪胰脏胰蛋白酶被应用于以下应用中：
  • 凝胶内蛋白消化及MALDI-TOF质谱分析
  • 电喷雾电离质谱(ESI-MS)分析
  • L. 植物杆菌的表面蛋白质组分析
  • 凝胶过滤，超离心，旋转阴影电子显微镜
  • 质谱

生化／生理作用

由于胰蛋白酶具有高度特异性裂解能力，胰蛋白酶肽数量有限，因此胰蛋白酶通常用于蛋白质组学研究中的肽定位和蛋白质序列研究。它可特异性地水解精氨酸和赖氨酸残基羧基侧的肽键。该酶还具有酯酶和酰胺酶的活性。胰蛋白酶作为一种细胞培养工具。在工业上，它被用来水解致敏蛋白以生产低致敏牛奶。

其他說明

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)

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说明
价格

象形圖

Health hazardExclamation mark
GHS08,GHS07

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 1

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Pauline Floch et al.
Gut pathogens, 6, 20-20 (2014-07-06)
A gamma-glutamyl transpeptidase (GGT) is produced by up to 31% of strains of Campylobacter jejuni isolates. C. jejuni GGT is close to Helicobacter pylori GGT suggesting a conserved activity but unlike the latter, C. jejuni GGT has not been studied
Yusuke Murasawa et al.
The Journal of biological chemistry, 288(40), 29170-29181 (2013-08-22)
Versican G1 domain-containing fragments (VG1Fs) have been identified in extracts from the dermis in which hyaluronan (HA)-versican-fibrillin complexes are found. However, the molecular assembly of VG1Fs in the HA-versican-microfibril macrocomplex has not yet been elucidated. Here, we clarify the role
Masayuki Shimoda et al.
Cell transplantation, 21(2-3), 465-471 (2012-07-17)
Porcine islets are considered to be a promising resource for xenotransplantation. However, it is difficult to isolate porcine islets because of the marked fragility and rapid dissociation. Endogenous trypsin is one of the main factors to damage islets during the
Hui Zheng et al.
Molecules (Basel, Switzerland), 18(11), 13425-13433 (2013-11-02)
A novel chemoenzymatic one-pot multicomponent synthesis of thiazole derivatives was developed. A series of thiazole derivatives were synthesized with high yields up to 94% under mild enzyme-catalyzed conditions. The blank and control experiments reveal that trypsin from porcine pancreas (PPT)
Maciej Suski et al.
Biochimica et biophysica acta, 1834(12), 2463-2469 (2013-08-31)
Excessive action of angiotensin II on mitochondria has been shown to play an important role in mitochondrial dysfunction, a common feature of atherogenesis and kidney injury. Angiotensin-(1-7)/Mas receptor axis constitutes a countermeasure to the detrimental effects of angiotensin II on
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实验方案

Procedure for Enzymatic Assay of Trypsin (EC 3.4.21.4)

This procedure is for products with a specification for Trypsin activity using Na-Benzoyl-L-arginine ethyl ester (BAEE) as a substrate. The procedure is a continuous spectrophotometric rate determination (A253, Light path = 1 cm).

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