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一般說明
采用方便的一次性1 μg包装,体验蛋白质组学级胰蛋白酶的所有优点。
蛋白质组学级胰蛋白酶单品具有出色的蛋白水解效率,可产生更多的胰蛋白酶肽,从而为您感兴趣的肽提供更大的序列覆盖率。由于干扰性自溶峰及其模糊加合物的数量减少,从而大大简化了质谱分析。目前已经从猪胰腺分离出大量蛋白质组学级胰蛋白酶,使其能够在Arg和Lys残基的羧酸侧实现精准切割。通过对酶进行彻底的还原性甲基化处理,能够最大限度地减少自溶,并且已通过TPCK处理淬灭了胰凝乳蛋白酶活性。通过亲和层析以及稀酸干燥等进一步纯化步骤,获得高纯度的高比活性胰蛋白酶,特别符合蛋白质组学研究的苛刻标准,并且专用于溶液或凝胶类消化物。
蛋白质组学级胰蛋白酶单品具有出色的蛋白水解效率,可产生更多的胰蛋白酶肽,从而为您感兴趣的肽提供更大的序列覆盖率。由于干扰性自溶峰及其模糊加合物的数量减少,从而大大简化了质谱分析。目前已经从猪胰腺分离出大量蛋白质组学级胰蛋白酶,使其能够在Arg和Lys残基的羧酸侧实现精准切割。通过对酶进行彻底的还原性甲基化处理,能够最大限度地减少自溶,并且已通过TPCK处理淬灭了胰凝乳蛋白酶活性。通过亲和层析以及稀酸干燥等进一步纯化步骤,获得高纯度的高比活性胰蛋白酶,特别符合蛋白质组学研究的苛刻标准,并且专用于溶液或凝胶类消化物。
應用
蛋白质组学级胰蛋白酶单品已被用于蛋白质消化,以通过MALDI-TOF MS(基质辅助激光解吸电离-飞行时间质谱法)分析产生的肽。它已被用于蛋白质消化,以通过LC-MS(液相色谱-质谱法)/MS分析产生的肽。
特點和優勢
- 简单方便,一次性1 μg用量
- 减少重复加样
- 还原性甲基化以减少自溶活性
- TPCK处理以消除胰凝乳蛋白活性
- 高度纯化
相關產品
产品编号
说明
价格
訊號詞
Danger
危險分類
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
3 - Flammable liquids
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