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Merck

T7575

Sigma-Aldrich

蛋白质组学级胰蛋白酶单品

别名:

Proteomics grade, Trypsin, TPCK treated

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.56

等級

Proteomics Grade

品質等級

形狀

ready-to-use solution

比活性

≥10,000 BAEE units/mg protein

分子量

average mol wt 23.29 kDa

最適pH

~8.0

儲存溫度

2-8°C

相关类别

一般說明

采用方便的一次性1 μg包装,体验蛋白质组学级胰蛋白酶的所有优点。

蛋白质组学级胰蛋白酶单品具有出色的蛋白水解效率,可产生更多的胰蛋白酶肽,从而为您感兴趣的肽提供更大的序列覆盖率。由于干扰性自溶峰及其模糊加合物的数量减少,从而大大简化了质谱分析。目前已经从猪胰腺分离出大量蛋白质组学级胰蛋白酶,使其能够在Arg和Lys残基的羧酸侧实现精准切割。通过对酶进行彻底的还原性甲基化处理,能够最大限度地减少自溶,并且已通过TPCK处理淬灭了胰凝乳蛋白酶活性。通过亲和层析以及稀酸干燥等进一步纯化步骤,获得高纯度的高比活性胰蛋白酶,特别符合蛋白质组学研究的苛刻标准,并且专用于溶液或凝胶类消化物。

應用

蛋白质组学级胰蛋白酶单品已被用于蛋白质消化,以通过MALDI-TOF MS(基质辅助激光解吸电离-飞行时间质谱法)分析产生的肽。它已被用于蛋白质消化,以通过LC-MS(液相色谱-质谱法)/MS分析产生的肽。

特點和優勢

  • 简单方便,一次性1 μg用量
  • 减少重复加样
  • 还原性甲基化以减少自溶活性
  • TPCK处理以消除胰凝乳蛋白活性
  • 高度纯化

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • Trypsin Solubilization Reagent

相關產品

产品编号
说明
价格

訊號詞

Danger

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

3 - Flammable liquids


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Molecular systems biology, 4, 180-180 (2008-04-17)
The human protein interaction network will offer global insights into the molecular organization of cells and provide a framework for modeling human disease, but the network's large scale demands new approaches. We report a set of 7000 physical associations among
Daniel W Udwary et al.
Applied and environmental microbiology, 77(11), 3617-3625 (2011-04-19)
Bacteria of the genus Frankia are mycelium-forming actinomycetes that are found as nitrogen-fixing facultative symbionts of actinorhizal plants. Although soil-dwelling actinomycetes are well-known producers of bioactive compounds, the genus Frankia has largely gone uninvestigated for this potential. Bioinformatic analysis of
Michael Costanzo et al.
Science (New York, N.Y.), 327(5964), 425-431 (2010-01-23)
A genome-scale genetic interaction map was constructed by examining 5.4 million gene-gene pairs for synthetic genetic interactions, generating quantitative genetic interaction profiles for approximately 75% of all genes in the budding yeast, Saccharomyces cerevisiae. A network based on genetic interaction
Tiziana Cabras et al.
Molecular & cellular proteomics : MCP, 9(10), 2099-2108 (2010-06-30)
The acidic soluble fraction of whole saliva of type 1 diabetic children was analyzed by reversed phase (RP)(1)-HPLC-ESI-MS and compared with that of sex- and age-matched control subjects. Salivary acidic proline-rich phosphoproteins (aPRP), histatins, α-defensins, salivary cystatins, statherin, proline-rich peptide
Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry.
Tripkovic T, et al.
Talanta, 113, 49-61 (2013)

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