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Merck

F8435

Sigma-Aldrich

糖苷酶F 来源于脑膜脓毒性伊丽莎白菌

lyophilized powder, recombinant, expressed in E. coli

别名:

N-糖苷酶F, 糖苷酶F 来源于脑膜炎脓毒性黄杆菌, 糖苷酶F 来源于脑膜脓毒性金黄杆菌, 肽N-糖苷酶

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

重組細胞

expressed in E. coli

品質等級

共軛

(N-linked)

等級

Proteomics Grade

形狀

lyophilized powder

比活性

≥1,000 U/mg

儲存期限

≥1 weeks at 2‑8 °C (for reconstituted solution)
≥1 yr at -20 °C

分子量

~36 kDa

運輸包裝

wet ice

儲存溫度

−20°C

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相关类别

應用

用于使蛋白质去糖基化。
高度纯化的材料可用于制备性去糖基化或在凝胶、溶液或印迹膜中用于分析应用。可通过利用其C-末端6x组氨酸融合标签从制备性操作中去除该酶。

生化/生理作用

从糖蛋白切割整个聚糖,前提是糖基化天冬酰胺部分在其氨基和羧基末端被多肽链取代。

包裝

在一项调查他汀类药物对p-糖蛋白的双重影响及其对人神经母细胞瘤细胞中阿霉素细胞毒性影响的研究中,PNGase F已被用于P-糖蛋白的去糖基化。它在一项研究中的定量免疫印迹分析前被用于处理纯化的蛋白,小鼠视锥紫外线(MUV)色素它已被用于对N-连接的糖蛋白进行糖基化。高度纯化的材料可用于制备性去糖基化或在凝胶、溶液或印迹膜中用于分析应用。可通过利用其C-末端6x组氨酸融合标签从制备性操作中去除该酶。

單位定義

一个单元将在37℃、pH7.5下通过SDS-PAGE监测,在1分钟内催化1纳摩尔变性核糖核酸酶B释放N-连接的寡糖。 PNGase F活性的一个 Sigma 单位等于1 IUB毫单位。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Maria Nordgren et al.
Methods in molecular biology (Clifton, N.J.), 2271, 155-167 (2021-04-29)
O-glycosylation is a difficult posttranslational modification to analyze. O-glycans are labile and often cluster making their analysis by LC-MS very challenging. OpeRATOR is an O-glycan specific protease that cleaves the protein backbone N-terminally of glycosylated serine and threonine residues. This
Lauren L Daniele et al.
Investigative ophthalmology & visual science, 46(6), 2156-2167 (2005-05-26)
To test the hypothesis that Nrl(-)(/)(-) photoreceptors are cones, by comparing them with WT rods and cones using morphological, molecular, histochemical, and electrophysiological criteria. The photoreceptor layer of fixed retinal tissue of 4- to 6-week-old mice was examined in plastic
T H Plummer et al.
The Journal of biological chemistry, 259(17), 10700-10704 (1984-09-10)
Endo-beta-N-acetylglucosaminidase F preparations from Flavobacterium meningosepticum have been found to contain peptide:N-glycosidase activity. Only the second activity, designated as peptide:N-glycosidase F, readily cleaves the beta-aspartylglycosylamine linkage of a fetuin triantennary complex glycopeptide, as shown by the isolation of the corresponding
Evelyn Sieczkowski et al.
International journal of cancer, 126(9), 2025-2035 (2009-09-10)
The development of multidrug resistance (MDR) is a major problem during cancer treatment. Drug efflux via ATP-binding cassette (ABC) transporters is the main mechanism responsible for resistance to chemotherapeutics. We have recently observed that statins enhance susceptibility to doxorubicin-induced apoptosis
Ulla-Maja Bailey et al.
Journal of proteome research, 11(11), 5376-5383 (2012-10-09)
Asparagine-linked glycosylation is a common post-translational modification of proteins in eukaryotes. Mutations in the human ALG3 gene cause changed levels and altered glycan structures on mature glycoproteins and are the cause of a severe congenital disorder of glycosylation (CDG-Id). Diverse

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