M3148

2-巯基乙醇

for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

• 别名: β-巯基乙醇, 2-羟乙基硫醇, BME, 硫代二甘醇
• 线性分子式: HSCH₂CH₂OH
• CAS号: 60-24-2
• 分子量: 78.13
• Beilstein: 773648
• EC號碼: 200-464-6
• MDL號碼: MFCD00004890
• 分類程式碼代碼: 12161504
• PubChem物質ID: 24896800
• NACRES: NA.31

属性

• 等級: Molecular Biology; for molecular biology
• 蒸汽密度: 2.69 (vs air)
• 蒸汽壓力: 1 mmHg ( 20 °C)
• 產品線: BioReagent
• 化驗: 99% (GC/titration)
• 形狀: liquid
• expl. lim.: 18 %
• 反應適用性: reagent type: reductant
• 濃度: 14.3 M (pure liquid)
• 技術: cell culture | mammalian: suitable; electrophoresis: suitable
• 折射率: n20/D 1.500 (lit.)
• pH值: 4.5-6 (20 °C, 500 g/L)
• bp: 157 °C (lit.)
• 溶解度: H₂O: soluble 1 mL/mL
• 密度: 1.114 g/mL at 25 °C (lit.)
• 異物活動: DNase, RNase, protease, none detected
• 儲存溫度: 2-8°C
• SMILES 字串: OCCS
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChI 密鑰: DGVVWUTYPXICAM-UHFFFAOYSA-N

说明

應用

还原剂
β-巯基乙醇或2-巯基乙醇已用于：

• 作为Roswell Park Memorial Institute (RPMI)-1640培养基的一种补充剂用于DT40细胞（鸡B细胞系）的培养
• 裂解NuLi-1（Normal Lung, University of Iowa-1）细胞
• 小鼠胚胎干细胞的胰蛋白酶消化

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

安全信息

• 象形圖: GHS06,GHS08,GHS05,GHS09
• 訊號詞: Danger
• 危險聲明: H301 + H331,H310,H315,H317,H318,H361f,H373,H410
• 防範說明: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• 危險分類: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• 標靶器官: Liver,Heart
• 儲存類別代碼: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: 154.4 °F - closed cup
• 閃點（°C）: 68 °C - closed cup
• 個人防護裝備: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter