444203

β巯基乙醇，分子生物学级

A disulfide-reducing agent.

• 别名: 2-巯基乙醇, BME, β-巯基乙醇, 2-羟乙基硫醇, 硫代二甘醇
• 线性分子式: HSCH₂CH₂OH
• CAS号: 60-24-2
• 分子量: 78.13
• Beilstein: 773648
• MDL號碼: MFCD00004890
• 分類程式碼代碼: 12161504
• NACRES: NA.25
• bp: 157 °C (lit.)

属性

• 等級: Molecular Biology
• 品質等級: 100
• 蒸汽密度: 2.69 (vs air)
• 蒸汽壓力: 1 mmHg ( 20 °C)
• 描述: Merck USA index - 14, 5869
• 化驗: ≥99% (GC)
• 形狀: liquid
• expl. lim.: 18 %
• 反應適用性: reagent type: reductant
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze
• 濃度: 14.3 M (pure liquid)
• 折射率: n20/D 1.500 (lit.)
• pH值: 4.5-6 (20 °C, 500 g/L)
• bp: 157 °C (lit.)
• mp: <-50 °C
• 密度: 1.12 g/cm³ at 20 °C; 1.114 g/mL at 25 °C (lit.)
• 正離子痕跡: heavy metals (as Pb): ≤0.0001%
• 吸收: ≤0.31 at 280 nm; ≤1.50 at 260 nm
• 異物活動: DNases, proteases, RNases, none detected
• 運輸包裝: ambient
• 儲存溫度: 15-25°C
• SMILES 字串: OCCS
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChI 密鑰: DGVVWUTYPXICAM-UHFFFAOYSA-N

说明

一般說明

二硫还原剂。

二硫还原剂。有机合成中常用的试剂（吸光度：≤260 nm处1.50；280 nm处≤0.31）。

應用

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

警告

毒性：有毒（F）

其他說明

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法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 象形圖: GHS06,GHS08,GHS05,GHS09
• 訊號詞: Danger
• 危險聲明: H301 + H331,H310,H315,H317,H318,H361f,H373,H410
• 防範說明: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• 危險分類: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• 標靶器官: Liver,Heart
• 儲存類別代碼: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: 165.2 °F - closed cup
• 閃點（°C）: 74 °C - closed cup