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Merck
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B5655

Sigma-Aldrich

BCIP®/NBT

SIGMAFAST™, alkaline phosphatase substrate, chromogenic, tablet

别名:

BCIP®/NBT碱性磷酸酶底物

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.83

product name

SIGMAFAST™ BCIP®/NBT, tablet

形狀

tablet

溶解度

water: 10 mL, clear, yellow

儲存溫度

−20°C

相关类别

應用

SIGMAFAST BCIP®/NBT已被用作以下底物：
  • 用于免疫印迹的抗小鼠IgG偶联碱性磷酸酶
  • 用于原代成骨细胞组织化学染色中碱性磷酸酶
  • 骨髓基质细胞碱性磷酸酶染色

重構

将一片溶于10 ml去离子水中，得到含有BCIP/NBT的即用型缓冲溶液，pH 9.5。

法律資訊

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 2

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Andriy Didovyk et al.
ACS synthetic biology, 6(12), 2198-2208 (2017-08-11)
Cell-free gene expression systems are emerging as an important platform for a diverse range of synthetic biology and biotechnology applications, including production of robust field-ready biosensors. Here, we combine programmed cellular autolysis with a freeze-thaw or freeze-dry cycle to create
Sialic acid on herpes simplex virus type 1 envelope glycoproteins is required for efficient infection of cells
Teuton JR and Brandt CR
Journal of Virology, 81(8), 3731-3739 (2007)
MicroRNA-183-5p increases with age in bone-derived extracellular vesicles, suppresses bone marrow stromal (stem) cell proliferation, and induces stem cell senescence
Davis C, et al.
Tissue Engineering: Part A, 23(21-22), 1231-1240 (2017)
Mitogen-activated protein kinase phosphatase 1 regulates bone mass, osteoblast gene expression, and responsiveness to parathyroid hormone
Mahalingam CD, et al.
The Journal of Endocrinology, 211(2), 145-156 (2011)
Aneela Yasmeen et al.
Molecular biotechnology, 58(12), 807-820 (2016-10-21)
The conserved coat or V2 gene of begomoviruses is responsible for viral movement in the plant cells. RNAi technology was used to silence V2 gene for resistance against these viruses in transgenic plants. The transformation of the RNAi-based gene construct
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商品

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

实验方案

Immunodetection Using BCIP/NBT Substrate

5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) is an ideal insoluble substrate for use with alkaline phosphatase. This system produces a blue-purple product. The intense color can be observed visually, is very stable, and will not fade upon exposure to light.

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