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Sigma-Aldrich

BCIP®/NBT

alkaline phosphatase substrate, chromogenic, liquid

别名:

5-bromo-4-chloro-3-indolyl-phosphate / Nitro blue tetrazolium chloride solution, BCIP/NBT Alkaline Phosphatase Substrate solution

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.83

product name

BCIP®/NBT液体底物系统, ready to use solution

品質等級

300

形狀

liquid

儲存溫度

2-8°C

相关类别

應用

BCIP®/NBT液体底物系统用于：
  • 对骨髓基质细胞中的碱性磷酸酶（ALP）进行染色
  • 用作Western 印迹检测的底物，检测基质金属蛋白酶-2特异性抗体
  • 作为酶联免疫斑点（ELISpot）测定中的底物，测定链霉抗生物素蛋白碱性磷酸酶

碱性磷酸酶的即用型沉淀底物系统。该底物系统能够产生不溶的NBT二甲双胍终产物，其颜色为蓝色至紫色并且可以在视觉上观察到。使用BCIP/NBT的免疫组织化学染色需要有机封固剂，并且可以用核固红或亮绿复染。BCIP/NBT通常比固红更灵敏。 不建议用于ELISA（微孔）检测。

注意

建议将本品储存在4-8°C之间。请勿冷冻。本品可在室温下储存最长一年。如果产生沉淀，可将溶液轻轻涡旋或过滤。

法律資訊

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

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Commensal microbes induce serum IgA responses that protect against polymicrobial sepsis
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The relationship between the antigen dose and the quality of an immune response generated upon immunization is poorly understood. However, findings show that the immune system is indeed influenced by the antigen dose; hence underlining the importance of correctly determining
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The Catsper1 gene, whose expression is restricted to male germ cells, has great importance in reproductive biology because of its function in sperm motility and fertilization. We previously reported that the promoter of this gene has transcriptional activity in either
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商品

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

实验方案

Immunodetection Using BCIP/NBT Substrate

5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) is an ideal insoluble substrate for use with alkaline phosphatase. This system produces a blue-purple product. The intense color can be observed visually, is very stable, and will not fade upon exposure to light.

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