跳转至内容
Merck
所有图片(3)

Key Documents

View All Documentation

B0274

Sigma-Aldrich

5-溴-4-氯-3-吲哚磷酸 对甲苯胺盐

tablet

别名:

BCIP® 对甲苯胺盐, X-磷酸盐 对甲苯胺盐

登录查看公司和协议定价
所有图片(3)

About This Item

经验公式（希尔记法）:
C8H6BrClNO4P · C7H9N
CAS号:
6578-06-9
分子量:
433.62
EC號碼:
229-506-1
MDL號碼:
MFCD00040636
分類程式碼代碼:
12352204
PubChem物質ID:
57653906
NACRES:
NA.83

形狀

tablet

品質等級

200

儲存溫度

−20°C

SMILES 字串

Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23

InChI

1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3

InChI 密鑰

QEIFSLUFHRCVQL-UHFFFAOYSA-N

正在寻找类似产品? 访问 产品对比指南

相关类别

特異性

Substrate of choice for use with alkaline phosphatase in immunoblotting and, less commonly, in immunohistological staining procedures. High assay sensitivity is achieved via amplification when BCIP is used in conjunction with Nitro Blue Tetrazolium (NBT) Tablets (Catalog No. N5514). This substrate produces an insoluble end product that is blue-purple in color and can be observed visually.

應用

用于碱性磷酸酶标记分子的比色检测，可使用5-溴-4-氯-3-吲哚基磷酸酯（BCIP）和硝基蓝四唑（NBT）。BCIP/NBT底物系统具有多种功能并用于多种应用，包括Northern Southern和Western blotting、原位杂交和免疫组化。
BCIP以两种盐形式提供：可溶于水的二钠盐和可溶于二甲基甲酰胺的对甲苯胺形式。这些盐形式可用于制备与NBT及反应缓冲液组合的储存溶液，并形成碱性磷酸酶的底物溶液。当与碱性磷酸酶一起孵育时，该底物系统可产生不溶的NBT二甲嗪产物，具有容易被观察到的紫色。为了方便起见，BCIP和NBT的混合物是以一种易溶解的片剂形式或作为即用型液体而提供的。

數量

Contains 25 mg substrate per tablet.

基底

碱性磷酸酶的组织化学底物。

法律資訊

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

其他客户在看

Slide 1 of 1

1 of 1

BCIP®/NBT液体底物系统 ready to use solution

Sigma-Aldrich

B1911

BCIP®/NBT液体底物系统

Carolyn Byrne et al.
Methods in molecular biology (Clifton, N.J.), 289, 127-136 (2004-10-27)
The skin as a surface organ is uniquely accessible for whole embryo/fetal analyzes of developmental changes, such as gene induction, protein expression, formation of epidermal-derived appendages such as hair follicles, and formation of the protective barrier. Such analyses have emphasized
M S Blake et al.
Analytical biochemistry, 136(1), 175-179 (1984-01-01)
A rapid, sensitive method has been developed to detect antibody-antigen complexes on "Western blots." The methods of H. Towbin, T. Staehlin, and J. Gordon were used to separate and blot the antigens onto nitrocellulose. The remaining sites of attachment were
Audrey Fouchs et al.
Biology of the cell, 102(8), 447-456 (2010-04-10)
Activation of MAPKs (mitogen-activated protein kinases), in particular ERK1/2 (extracellular-signal-regulated kinase 1/2), has been reported to take place in a large variety of cell types after hypo-osmotic cell swelling. Depending on cell type, ERK1/2 phosphorylation can then serve or not
Substrates for cytochemical demonstration of enzyme activity. II. Some dihalo-3-indolyl phosphates and sulfates.
J P Horwitz et al.
Journal of medicinal chemistry, 9(3), 447-447 (1966-05-01)
Willemijn A van Dop et al.
Gastroenterology, 139(5), 1665-1676 (2010-08-05)
Indian Hedgehog (Ihh) is expressed by the differentiated epithelial cells of the small intestine and signals to the mesenchyme where it induces unidentified factors that negatively regulate intestinal epithelial precursor cell fate. Recently, genetic variants in the Hh pathway have
查看所有相关文献

商品

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门