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B8503

Sigma-Aldrich

5-溴-4-氯-3-吲哚磷酸 对甲苯胺盐

chromogenic, ≥99% (HPLC), powder

别名:

BCIP® 对甲苯胺盐, X-磷酸盐 对甲苯胺盐

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About This Item

经验公式（希尔记法）:
C8H6BrClNO4P · C7H9N
CAS号:
6578-06-9
分子量:
433.62
EC號碼:
229-506-1
MDL號碼:
MFCD00040636
分類程式碼代碼:
12352204
PubChem物質ID:
57653963
NACRES:
NA.83

product name

5-溴-4-氯-3-吲哚磷酸 对甲苯胺盐, ≥99% (HPLC)

品質等級

200

化驗

≥99% (HPLC)

形狀

powder

溶解度

DMF: 20 mg/mL, clear to slightly hazy, yellow

儲存溫度

−20°C

SMILES 字串

Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23

InChI

1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3

InChI 密鑰

QEIFSLUFHRCVQL-UHFFFAOYSA-N

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相关类别

一般說明

5-溴-4-氯-3-吲哚基磷酸酯（BCIP）是一种组织化学磷酸酶染料。

應用

5-溴-4-氯-3-吲哚基磷酸酯对甲苯胺盐已被用于原位杂交。
用于碱性磷酸酶标记分子的比色检测，可使用5-溴-4-氯-3-吲哚基磷酸酯（BCIP）和硝基蓝四唑（NBT）。BCIP/NBT底物系统具有多种功能并用于多种应用，包括Northern Southern和Western blotting、原位杂交和免疫组化。
BCIP以两种盐形式提供：可溶于水的二钠盐和可溶于二甲基甲酰胺的对甲苯胺形式。这些盐形式可用于制备与NBT及反应缓冲液组合的储存溶液，并形成碱性磷酸酶的底物溶液。当与碱性磷酸酶一起孵育时，该底物系统可产生不溶的NBT二甲嗪产物，具有容易被观察到的紫色。为了方便起见，BCIP和NBT的混合物是以一种易溶解的片剂形式或作为即用型液体而提供的。

生化／生理作用

5-溴-4-氯-3-吲哚磷酸酯（BCIP）可用于对磷酸酶活性成像。它可用作底物，监控溶液中的分泌性胚胎碱性磷酸酶活性。

包裝

无底玻璃瓶。内含物装在插入的融合锥内。

基底

碱性磷酸酶的组织化学底物。

法律資訊

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves

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SEAP activity measurement in reporter cell-based assays using BCIP/NBT as substrate
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Journal of Lipid Research, 46(11), 2339-2346 (2005)
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Wasting marmoset syndrome (WMS) is a serious disease in captive common marmoset (Callithrix jacchus) colonies. Because of the high mortality rates, elucidation of the underlying mechanisms is essential. In this study, we compared the histopathology, the number of each epithelial
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商品

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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