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一般說明
抗生物素蛋白 (Avidin) 是一种四聚体或二聚体的生物素结合蛋白，产生于鸟类、爬行动物和两栖动物的输卵管中，并沉积在其蛋清中。由生物素的 4 个高亲和力结合位点组成。ExtrAvidin ® 由蛋清亲和素制备而成。它是亲和纯化亲和素的改良形式，具有亲和素的高比活性和链霉亲和素的低背景染色。是一种由 阿维迪链霉菌 产生的生物素结合蛋白。ExtrAvidin已与碱性磷酸酶偶联，可用于多种技术。
應用
ExtrAvidin ™-碱性磷酸酶已被用于：
- 斑点杂交法
- 免疫组化（福尔马林固定、石蜡包埋切片）
- 间接 ELISA
- 酶联免疫吸附试验 (ELISA)
- 酶联免疫吸附点 (ELISPOT) 分析
- 竞争性ELISA
生化／生理作用
碱性磷酸酶催化酯水解生成磷酸。 它还促进物质的跨膜运输。
外觀
含有1 mM MgCl2、1% 牛血清白蛋白和15 mM 叠氮化钠的 0.05 M Tris-HCl 缓冲液，pH 8.0中的溶液
準備報告
亲和纯化蛋白
法律資訊
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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商品
The enzyme-linked immunosorbent spot (ELISpot ) assay enables visualization of multiple secretory products from a single responding cell. The ELISpot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information.
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