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應用
SIGMA FAST p-磷酸硝基苯酯片剂用于：
- 定量非分泌型碱性磷酸酶，监测成骨样细胞的分化情况（SaoS-2）
- 染色小鼠前成骨细胞 MC3T3-E1，进行碱性磷酸酶测定
- 用作 ExtrAvidin 碱性磷酸酶的酶作用物®，通过酶联免疫吸附法（ELISA）测定血红蛋白结合α-突触核蛋白
- 用作碱性磷酸酶偶联抗体的酶作用物，进行Abirin毒素的ELISA检测
开发用作酶免疫分析 (EIA) 中检测碱性磷酸酶活性的可溶性底物。pNPP 是基于高灵敏度碱性磷酸酶的 EIA 检测中的首选底物。将 1 片 pNPP 片剂和 1 片 Tris 缓冲液片剂溶于去离子水中可作为即用型底物。各 Sigma 快速 pNPP 片剂组得到的溶液含 1.0 mg/mL pNPP，0.2 MTris 缓冲液。使用 pNPP 的 EIA 应用可在定时测定中读取，或用 3N NaOH 停止以延迟读取。SIGMA 快速 ™ pNPP仅需要添加蒸馏水或去离子水，即可制备活性底物溶液。片剂单独包装在铝箔包装中 。
重構
将一组片剂溶于5 mL去离子水中，即可得到含有pNPP的即用型缓冲液。
法律資訊
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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商品
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.
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