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N7653

Sigma-Aldrich

对硝基苯磷酸盐液体底物系统

alkaline phosphatase substrate, liquid

别名:

4-硝基苯磷酸二钠 溶液, pNPP

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About This Item

线性分子式:
O2NC6H4OP(O)(ONa)2
CAS号:
4264-83-9
分子量:
263.05
MDL號碼:
MFCD00007319
分類程式碼代碼:
12352204
PubChem物質ID:
24897835
NACRES:
NA.83

product name

对硝基苯磷酸盐液体底物系统, liquid

形狀

liquid

運輸包裝

wet ice

儲存溫度

−20°C

SMILES 字串

[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

InChI 密鑰

VIYFPAMJCJLZKD-UHFFFAOYSA-L

相关类别

一般說明

对硝基苯磷酸盐（pNPP）液体底物系统将对硝基苯磷酸盐、缓冲液和所需的镁阳离子结合在一起，成为一种便捷的即用型单一溶液试剂。pNPP能够产生可溶性终产物，是碱性磷酸酶免疫测定的理想底物。

應用

在碱性磷酸酶测定中，采用p-对硝基苯基磷酸盐液体底物体系作为测定碱性磷酸酶活性的底物。
碱性磷酸酶的底物，能够产生可在405 nm处读取的可溶性黄色终产物。使用3N NaOH可终止反应并在405 nm处读取结果。推荐用于ELISA（微孔）分析，不建议用于膜应用。

生化／生理作用

4-硝基苯基磷酸二钠盐六水(pNPP)是测定蛋白质酪氨酸磷酸酶活性的非蛋白质和非特异性底物。pNPP水解后会生成p-硝基酚。它是碱性磷酸酶常见的显色底物。

注意

可在低于0°C的环境中稳定储存至少一年。

外觀

即用型。

象形圖

Health hazardCorrosion
GHS08,GHS05

訊號詞

Danger

危險分類

Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

標靶器官

Kidney,Liver,Blood

儲存類別代碼

8B - Non-combustible corrosive hazardous materials

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析证书(COA)

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双（对硝基苯基）磷酸盐 钠盐 ≥99% (TLC)

Sigma-Aldrich

N3002

双（对硝基苯基）磷酸盐 钠盐

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