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D4418

Sigma-Aldrich

3,3′-Diaminobenzidine

SIGMAFAST™, chromogenic, tablet

别名:

DAB, DAB tablets, Immunohistology substrate

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About This Item

CAS号:
91-95-2
MDL號碼:
MFCD00007725
分類程式碼代碼:
12352204
PubChem物質ID:
24893872
NACRES:
NA.83

product name

SIGMAFAST™ 3,3′-二氨基联苯胺片, tablet, To prepare 15 mL

品質等級

200

形狀

tablet

溶解度

water: 1 tablet/5 mL

儲存溫度

−20°C

SMILES 字串

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI 密鑰

HSTOKWSFWGCZMH-UHFFFAOYSA-N

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應用

开发用于免疫组织学和免疫印迹，作为过氧化物酶活性定位的沉淀底物。DAB 是免疫组织学的首选底物，因为其可产生对酒精具有抗性的强烈棕黑色染料。用 DAB 染色的载玻片可脱水，封片于树脂介质中，并储存以备将来引用。SIGMAFAST™ DAB 片剂不需要额外的缓冲液或步骤来制备活性底物溶液。片剂单独包装在铝箔包装中

重構

将每片片剂溶于 15 mL 去离子水中，得到含 DAB 和过氧化氢尿素的即用型缓冲液。

法律資訊

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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E N Scully et al.
Behavioural brain research, 393, 112775-112775 (2020-06-23)
In both humans and animals, biological differences between males and females has long been a topic of research. In songbirds, sexual dimorphisms can be seen in many species' plumage and heard in some species' songs. However, not all songbirds have
Erin N Scully et al.
Behavioural brain research, 331, 151-158 (2017-05-17)
Zebra finches (Taeniopygia guttata) are sexually dimorphic songbirds, not only in appearance but also in vocal production: while males produce both calls and songs, females only produce calls. This dimorphism provides a means to contrast the auditory perception of vocalizations
F Gualtieri et al.
Scientific reports, 9(1), 7129-7129 (2019-05-11)
In the mammalian brain, adult hippocampal neurogenesis (AHN) is suppressed by chronic stress, primarily at the ventral pole of the hippocampus. Based upon anatomy, we hypothesise that the caudal pole of the avian Hippocampal Formation (HF) presents a homologous subregion.
Huiming Yang et al.
Nature communications, 11(1), 2582-2582 (2020-05-24)
Polyglutamine expansion in proteins can cause selective neurodegeneration, although the mechanisms are not fully understood. In Huntington's disease (HD), proteolytic processing generates toxic N-terminal huntingtin (HTT) fragments that preferentially kill striatal neurons. Here, using CRISPR/Cas9 to truncate full-length mutant HTT
Monika Verma et al.
Toxicon : official journal of the International Society on Toxinology, 139, 58-65 (2017-09-19)
Anthrax associated causalities in humans and animals are implicated mainly due to the action of two exotoxins that are secreted by the bacterium Bacillus antharcis during the infection. These exotoxins comprise of three protein components namely protective antigen (PA), lethal
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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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