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Merck

A9292

Sigma-Aldrich

兔抗马 IgG(全分子)抗体,偶联过氧物酶 兔抗

IgG fraction of antiserum, buffered aqueous solution

别名:

Rabbit Anti-Horse IgG (whole molecule)−HRP

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

rabbit

共軛

peroxidase conjugate

抗體表格

IgG fraction of antiserum

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

技術

direct ELISA: 1:15,000
dot blot: 1:100,000-1:200,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

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一般說明

Horse IgGs have seven subclasses ranging from IgG1 to IgG7. Equine IgG antibodies mainly regulate mucosal and systemic immunological responses and thereby, provide protection against disease-causing pathogens such as Streptococcus equi, and the horse flu virus. Horse IgG may also function to control the advancement of EHV-1 infection . Anti-horse IgG (whole molecule)−peroxidase antibody is specific for IgG in horses.

免疫原

Horse IgG

應用

Anti-horse IgG (whole molecule)−peroxidase antibody may be used in dot blot (1:8,000-1:10,000), immunohistochemistry (formalin-fixed, paraffin-embedded sections, at 1:150 dilution) and direct ELISA (1:15,000).
The level of S2-specific antibodies in test samples of horse serum was determined by ELISA using HRP-conjugated rabbit anti-horse IgG at a 1:35000 dilution with incubation for 45 minutes at room temperature.

生化/生理作用

IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab (antigen-binding fragments) fragments or bivalent F(ab′)2 fragments.  These two enzymes have a broad substrate specificity resulting in heterogenous fragments.

外觀

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

準備報告

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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