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Merck

A3688

Sigma-Aldrich

抗-小鼠 IgG(全分子)-碱性磷酸酶 山羊抗

affinity isolated antibody, buffered aqueous glycerol solution

别名:

Goat Anti-Mouse IgG (whole molecule)−AP

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

goat

共軛

alkaline phosphatase conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous glycerol solution

物種活性

mouse

應無反應活性

human

技術

direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

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一般說明

免疫球蛋白G(IgG)是一种糖蛋白抗体,其可调节免疫反应,如吞噬作用,也可参与自身免疫疾病的发展。小鼠IgG具有四种不同的同种型,即IgG1、IgG2a、IgG2b和IgG3。IgG1可调节小鼠的补体结合。
山羊抗小鼠IgG(全分子)-碱性磷酸酶抗体使用IEP与正常小鼠血清和小鼠IgG反应。通过ODD法,抗血清与小鼠IgG1、IgG2a、IgG2b、IgG3、IgA和IgM反应。通过ELISA,偶联物显示与人血清蛋白无反应性。

免疫原

纯化小鼠IgG

應用

使用碱性磷酸酶偶联的山羊抗小鼠IgG作为次级抗体,在37℃下1小时,通过蛋白质印迹分析人和鼠肿瘤细胞裂解液的各种蛋白表达。
在含有终浓度为0.5M NaCl的TBS/Tween中,使用碱性磷酸酶缀合的山羊抗小鼠IgG(μg/ ml)作为二抗,对支气管肺泡液中的表面活性蛋白A进行检测。
山羊抗小鼠IgG(全分子)-碱性磷酸酶抗体可用于免疫细胞化学分析和ELISA。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
蛋白质免疫印迹分析(1篇论文)

其他說明

与人血清蛋白吸附的抗体。

外觀

在0.05 M Tris(pH 8.0)中的溶液,含1%牛血清白蛋白、10 mM甘氨酸、1 mM MgCl2、50%甘油和15 mM叠氮化钠。

準備報告

经过吸附以减少人体样本的背景染色。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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访问文档库

Catharina E Dam et al.
Scandinavian journal of clinical and laboratory investigation, 74(6), 506-514 (2014-05-06)
Knowledge about antibody-antigen interactions is important for the understanding of the immune system mechanisms and for supporting development of drugs and biomarkers. A tool for identification of these antigenic epitopes of specific antibodies is epitope mapping. In this study, a
Alberta Bergamo et al.
The Journal of pharmacology and experimental therapeutics, 305(2), 725-732 (2003-02-28)
We have examined the biological and antitumor activity of a series of dinuclear ruthenium complexes. The aim of this study was to compare the in vitro effects of these new compounds on cell proliferation, cell distribution among cell cycle phases
Zahir Ali et al.
ACS synthetic biology, 11(1), 406-419 (2021-12-24)
Simple, rapid, specific, and sensitive point-of-care detection methods are needed to contain the spread of SARS-CoV-2. CRISPR/Cas9-based lateral flow assays are emerging as a powerful alternative for COVID-19 diagnostics. Here, we developed Bio-SCAN (biotin-coupled specific CRISPR-based assay for nucleic acid
J P Donahue et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(25), 12178-12182 (1994-12-06)
We have developed a method for crystallizing small functional protein segments so that their three-dimensional structure can be determined by x-ray diffraction analysis. This method consists of linking a small protein segment of unknown tertiary structure to either the amino
Sandra Alejandra Serna-Salas et al.
BioMed research international, 2018, 4706976-4706976 (2019-01-16)
Regulation of the mechanisms of fibrosis is an important goal in the treatment of liver cirrhosis. One mechanism is the participation of hepatic stellate cells in fibrogenesis when activated by catecholamines. Consequently, α/β adrenoblockers are proposed as an alternative treatment

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