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GC Analysis of Bacterial Acid Methyl Esters (BAMEs) on SUPELCOWAX™ 10 (15 m x 0.10 mm I.D., 0.10 μm), Fast GC Analysis

GC Analysis of Bacterial Acid Methyl Esters (BAMEs) on SUPELCOWAX™ 10 (15 m x 0.10 mm I.D., 0.10 μm), Fast GC Analysis suitable for GC

Materials

analytical column

Product No.
Description
Pricing

24343

SUPELCOWAX™ 10 Capillary GC Column

L × I.D. 15 m × 0.10 mm, df 0.10 μm

Standard

Product No.
Description
Pricing

47080-U

Bacterial Acid Methyl Ester (BAME) Mix

solution (10 mg/mL total concentration in methyl caproate), analytical standard

CONDITIONS

column

SUPELCOWAX 10, 15 m x 0.10 mm I.D., 0.10 μm (24343)

oven

80°C (0.3 min), 30 °C/min to 180 °C (0.5 min), 10 °C/min to 200 °C (1 min)

inj. temp.

250 °C

detector

FID, 250 °C

carrier gas

hydrogen, 45 cm/sec, constant

injection

0.1 μL 200:1 split

liner

2 mm I.D., straight

sample

bacterial methyl ester standards in methyl caproate, 10 mg/mL (47080-U)

Description

Analysis Note

Food-borne microbial pathogens can cause illness in humans. Because each bacterium has a unique cellular fatty acid profile, GC analysis can be used to help with identification. Analysis is performed following derivatization of cellular fatty acids to methyl esters. These are called bacterial acid methyl esters (BAMEs) instead of fatty acid methyl esters (FAMEs) to signify the bacterial source. The chromatogram shown is the analysis of a standard mix containing 26 BAME compounds. The polar nature of the column, along with the use of Fast GC column dimensions and instrument conditions, allows good peak shape and resolution in just 7 minutes.

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