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TOYOPEARL® HW-40S Bulk Media

phase hydroxylated methacrylate, bottle of 250 mL, 30 μm particle size

Synonym(s):

TOYOPEARL® HW-40 Size Exclusion Media

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About This Item

UNSPSC Code:
41115700

description

HW-40S

product line

TOYOPEARL®

form

slurry

packaging

bottle of 250 mL

parameter

3 bar max. pressure

technique(s)

HPLC: suitable

matrix

hydroxylated methacrylic polymer

matrix active group

hydroxylated methacrylate phase

particle size

30 μm

pore size

100-7000 Da MW range

operating pH

2-13

separation technique

size exclusion (SEC)

storage temp.

2-8°C

General description

Toyopearl HW-40 resin is a hydroxylated methacrylic polymer for size exclusion chromatography. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da.

Application

TOYOPEARL® media fractionate mixtures of proteins and other large molecular weight compounds over a wide size range. Applications include separating RNA from protein, resolving oligosaccharides by degree of polymerization, and isolating agglutinin while maintaining a high hemagglutination titer.

Specifications

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Physical form

Shipped in 20% (v/v) ethanol.

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Legal Information

Toyopearl is a registered trademark of Tosoh Corporation

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

wgk_germany

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

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M V Pokrovskaya et al.
Protein expression and purification, 82(1), 150-154 (2012-01-10)
We have cloned ansB (YPTB1411) gene from Yersinia pseudotuberculosis Q66CJ2 and constructed stable inducible expression system that overproduce L-asparaginase from Y. pseudotuberculosis (YpA) in Escherichiacoli BL21 (DE3) cells. For purification of YpA we used Q-Sepharose and DEAE-Toyopearl column chromatography. We
Shu-fen Xu et al.
Fitoterapia, 83(1), 153-160 (2011-11-01)
Persimmon proanthocyanidin was fractionated on Toyopearl TSK-HW-50-F to yield a fraction with strong inhibition on the catalytic activity and edema-inducing activity and lethality of Chinese cobra PLA(2). Thiolysis suggested that the terminal units included C, EGCG and myricetin, and epicatechin
Olga N Smol'kina et al.
Carbohydrate research, 347(1), 161-163 (2011-11-22)
A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was

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