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Key Documents

SRP0184

Sigma-Aldrich

Aha1 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym(s):

AHA1, AHSA1, Activator of heat shock 90kDa protein ATPase homolog 1

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

38 kDa

packaging

pkg of 200 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

>0.02 mg/mL

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... AHSA1(10598)

General description

Human recombinant full-length AHA1 with C-terminal HIS tag (GenBank Accession No. NM_012111), expressed in E. coli. MW = 38 kDa,

Application

ATPase activity assay for Hsp90alpha.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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