Skip to Content
MilliporeSigma
All Photos(4)

Key Documents

R1755

Sigma-Aldrich

Rhodamine B isothiocyanate

mixed isomers, BioReagent, suitable for protein labeling

Synonym(s):

RBITC

Sign Into View Organizational & Contract Pricing


About This Item

Empirical Formula (Hill Notation):
C29H30ClN3O3S
CAS Number:
Molecular Weight:
536.08
EC Number:
MDL number:
UNSPSC Code:
12352116
PubChem Substance ID:
NACRES:
NA.32

description

labeling efficiency with bovine albumin > 70%

Quality Level

product line

BioReagent

fluorescence

λex 543 nm; λem 580 nm in methanol

suitability

suitable for protein labeling

storage temp.

−20°C

SMILES string

[Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccc(cc4C(O)=O)N=C=S)=[N+](\CC)CC)c1

InChI

1S/C29H29N3O3S.ClH/c1-5-31(6-2)20-10-13-23-26(16-20)35-27-17-21(32(7-3)8-4)11-14-24(27)28(23)22-12-9-19(30-18-36)15-25(22)29(33)34;/h9-17H,5-8H2,1-4H3;1H

InChI key

YVSWPCCVTYEEHG-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

Rhodamine B isothiocyanate (RhBITC) is an organic fluorescence dye and is widely used as a fluorescent tag in a variety of biological applications. RhBITC is adsorbed onto Ag on silica or polystyrene beads. It exhibits not only a strong surface-enhanced Raman scattering (SERS) signal but also a measurable amount of fluorescence. The RhBITC-modified Ag-deposited silica or polystyrene beads disperse well in ethanol. They are also readily coated in water with polyelectrolytes for their further derivatization with biological molecules of interest that can bind to target molecules.

Application

Rhodamine B isothiocyanate is used for the following applications:
  • Rhodamine isothiocyanate labeling
  • Rhodamine-labeled synthetic APF
  • Preparations of protein and fluorescent conjugates
  • Fluorescent red dye

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Jun-Sung Kim et al.
Journal of veterinary science, 7(4), 321-326 (2006-11-16)
Biocompatible silica-overcoated magnetic nanoparticles containing an organic fluorescence dye, rhodamine B isothiocyanate (RITC), within a silica shell [50 nm size, MNP@SiO2(RITC)s] were synthesized. For future application of the MNP@SiO2(RITC)s into diverse areas of research such as drug or gene delivery
S K Williams et al.
Proceedings of the National Academy of Sciences of the United States of America, 78(4), 2393-2397 (1981-04-01)
Microvessels isolated from rat epididymal fat exhibit differential vesicular ingestion rates for unmodified and non-enzymatically glycosylated rat albumin. While unmodified rat albumin is excluded from ingestion by endothelial micropinocytic vesicles, glycosylated albumin is avidly taken up by endocytosis. Interaction of
Control Over Aragonite Crystal Nucleation and Growth: An In Vitro Study of Biomineralization.
Yael Levi et al.
Chemistry (Weinheim An Der Bergstrasse, Germany), 4 (1998)
Kwan Kim et al.
Biosensors & bioelectronics, 24(7), 1864-1869 (2008-11-05)
Rhodamine B isothiocyanate (RhBITC) is a prototype dye molecule that is widely used as a fluorescent tag in a variety of biological applications. We report in this work that once RhBITC is adsorbed onto Ag on silica or polystyrene beads
Mengying Zhang et al.
Lab on a chip, 10(9), 1199-1203 (2010-04-15)
We report a simple methodology to fabricate PDMS multi-layer microfluidic chips. A PDMS slab was surface-treated by trichloro (1H,1H,2H,2H-perfluorooctyl) silane, and acts as a reusable transferring layer. Uniformity of the thickness of the patterned PDMS layer and the well-alignment could

Articles

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service