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Key Documents

MBD0064

Sigma-Aldrich

ExoPolyzyme

For enzymatic lysis of extracellular biofilm matrix

Synonym(s):

Biofilm degrading enzyme mix, Extracellular matrix degrading enzymatic mixture

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About This Item

UNSPSC Code:
42312305
NACRES:
NA.32

Quality Level

form

solid

packaging

pkg of 1 vial

shipped in

dry ice

storage temp.

-10 to -25°C

General description

Biofilms are complex structures, which adhere to surfaces and comprise of one or more types of bacteria and fungi. They form an extracellular matrix (ECM) which is generally composed of secreted polymers such as exopolysaccharides (EPS), extracellular DNA (eDNA), peptides and lipids. ExoPolyzyme is a mixture of seven enzymes that break down different components residing in ECM, such as EPS’s and lipids. Each of these enzymes has been previously used to inhibit or remove biofilm. The original development was in collaboration with Scott Tighe and the Extreme Microbiome Project.

Application

ExoPolyzme can be used in biofilm research and as a gentler method for removing the EPS and lipids from the ECM in metagenomic protocols, providing better access to the bacterial cells within the biofilm and eDNA.

Features and Benefits

  • Mixture of seven enzymes that break down different components residing in ECM, such as EPS’s and lipids
  • Gently removes the EPS and lipids from the ECM in metagenomic protocols
  • Allows better access to the bacterial cells within the biofilm and eDNA

Components

The components in ExoPolyzyme (MBD0064) are:




  • α-Amylase
  • Cellulase
  • β-Glucosidase
  • Lyticase
  • Alginate lyase
  • Lipase
  • Hemicellulase

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


Certificates of Analysis (COA)

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