cell culture | mammalian: suitable
(1) 3.2, (2) 6.5
H2O: 4 mL/vial, clear to slightly hazy, blue to purple (Stock solutions should be filter sterilized and stored at 2-8 °C in the dark.)
DNA synthesis | interferes
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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
M4287 has been cell culture tested; M0503 has not been cell culture tested.
Solutions of this product should not be stored frozen. Solutions can be stored at 2-8°C, and should be used within 1-2 weeks. Solutions are light sensitive, but the powder is not.
Mitomycin C is soluble at 0.5 mg/mL in water, yielding a clear blue solution.
Treatment of the feeder layer with media containing 100 micrograms/mL mitomycin at 37°C for 30 minutes blocked replication of ES cells.
A concentration between 10-50 micrograms/mL is typically used.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Ask a Scientist here.
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
This protocol describes a method for culturing TS cell lines. These cells can then be used to study trophoblast differentiation and placental function.
This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.