MAK436

AF Cholesterol Assay Kit

Sufficient for 100 colorimetric or fluorometric tests

• Synonym(s): Cholesterol Quantitation Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 100
• application(s): pharmaceutical
• detection method: colorimetric; fluorometric
• relevant disease(s): cardiovascular diseases, depression; cancer; neurological disorders
• storage temp.: −20°C

Description

General description

Cholesterol is a sterol and lipid present in the cell membranes, and is transported in the bloodstream of all animals. It is used to form cell membranes and hormones, and plays important roles in cell signaling processes. Elevated levels (hypercholesterolemia) have been associated with cardiovascular diseases such as atherosclerosis; whereas, low levels (hypocholesterolemia) may be linked to depression, cancer and cerebral hemorrhage.

Application

The AF Cholesterol Assay Kit may be used for:

• Cancer Research
• Cardiovascular Research
• Neurological Research

Features and Benefits

Efficient and Precise: Detect Cholesterol levels ranging from 0.1 to 10 mg/dL (colorimetric) and 0.02 to 2 mg/dL (fluorimetric) in a 30 minute room temperature reaction, providing both convenience and sensitivity.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Suitability

The kit is suitable for the quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism in serum, plasma etc.

Principle

Simple, direct and automation-ready procedures for measuring cholesterol are very desirable. The AF Cholesterol Assay Kit uses a single working reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at A₅₇₀ nm or fluorescence intensity at λ_Ex = 530 nm/λ_Em = 585 nm is directly proportional to the total cholesterol concentration in the sample.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Safety Information

• pictograms: GHS02,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H225,H315,H318,H336,H410
• pcodes: P210 - P233 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3
• target_organs: Central nervous system
• Storage Class: 3 - Flammable liquids
• flash_point_f: 71.6 °F
• flash_point_c: 22 °C