MilliporeSigma
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C4951

Sigma-Aldrich

Cholesterol-Water Soluble

powder, BioReagent, suitable for cell culture

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Synonym(s):
Cholesterol–methyl-β-cyclodextrin
NACRES:
NA.75

Quality Level

200

biological source

ovine wool

product line

BioReagent

form

powder

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 300 mg/mL

shipped in

ambient

storage temp.

−20°C

Application

Cholesterol-Water Soluble has been used:
  • in free cholesterol (FC) overloading of rat smooth muscle cells (SMCs)
  • in cytotoxicity assay
  • for the incubation of CD4+ T cells with Helicobacter pylori
  • to study the membrane cholesterol level in SH-SY5Y human neuroblastoma cells
  • to study the effects of cholesterol on cell viability and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction in primary cultured rat hippocampal neurons
  • as a supplement for growth of bacterial culture
  • to examine the interaction of S protein with angiotensin-converting enzyme 2 (ACE2)
  • to investigate the effects of cholesterol on potassium currents in the inner hair cells of guinea pigs
  • for reversible inhibition of proteinase K

Packaging

30 mg in poly bottle
Package size based on cholesterol

Other Notes

Contains approx. 40 mg of cholesterol per gram; balance methyl-β-cyclodextrin.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How much cholesterol will I be getting in the 30 mg package of Product C4951, Cholesterol-Water Soluble?

    The package size is based on the amount of cholesterol present.  There is approximately 40 mg of cholesterol per gram of material, the balance being Methyl-β-cyclodextrin.

  4. What is the molecular weight of Product C4951, Cholesterol-Water Soluble?

    386.65 g/mol

  5. How do I calculate the molarity of Product C4951, Cholesterol-Water Soluble?

    Calculate the molarity based on the cholesterol content of your solution.  For instance, if you dissolve 10 mg of material in water, approximately 0.4 mg of that 10 mg is cholesterol.

  6. What concentration of Product C4951, Cholesterol-Water Soluble, is used in the cell culture test?

    The water soluble cholesterol is tested for cell culture use at approximately 7.6 micrograms per/L.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Nicole Stichling et al.
PLoS pathogens, 14(3), e1006914-e1006914 (2018-03-10)
Macrophages are a diverse group of phagocytic cells acting in host protection against stress, injury, and pathogens. Here, we show that the scavenger receptor SR-A6 is an entry receptor for human adenoviruses in murine alveolar macrophage-like MPI cells, and important
Autophagy plays a protective role in free cholesterol overloading induced smooth muscle cells death
Xu K, et al.
Journal of Lipid Research, jlr-M005702 (2010)
Cholesterol does not affect the toxicity of amyloid beta fragment but mimics its effect on MTT formazan exocytosis in cultured rat hippocampal neurons
Abe K and Saito H
Neuroscience Research, 35(3), 165-174 (1999)
A novel reciprocal and biphasic relationship between membrane cholesterol and beta-secretase activity in SH-SY5Y cells and in human platelets
Liu WW, et al.
Journal of Neurochemistry, 108(2), 341-349 (2009)
Barbora Melkes et al.
Naunyn-Schmiedeberg's archives of pharmacology, 389(12), 1289-1300 (2016-09-08)
There are some indications that biased μ-opioid ligands may diversely affect μ-opioid receptor (MOR) properties. Here, we used confocal fluorescence recovery after photobleaching (FRAP) to study the regulation by different MOR agonists of receptor movement within the plasma membrane of
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