M2279

Minimum Essential Medium Eagle

With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): EMEM, MEM
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: phenol red: yes; sodium pyruvate: no; L-glutamine: no; HEPES: no; NaHCO₃: yes; Earle’s salts (5% CO₂): yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. It includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for the cultivation of a wide variety of cells grown in monolayers and attached cell lines, such as fibroblasts, in the presence of fetal bovine serum (FBS), calf, or horse sera.

Application

Minimum Essential Medium Eagle has been used to culture the:

• explants of aortic valve leaflets
• human liver cancer cell line (HepG2) cells
• human fetal brain progenitor-derived astrocyte cells (PDA)
• breast cancer cell line (MCF-7)
• human glioblastoma cell line (T98G)

Preparation Note

Supplement with 0.292 g/L L-glutamine.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable