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MilliporeSigma
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Key Documents

L6715

Sigma-Aldrich

Biocoll

liquid, sterile-filtered, BioReagent, suitable for hybridoma

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About This Item

UNSPSC Code:
12352207

sterility

sterile-filtered
sterile

Quality Level

product line

BioReagent

form

liquid

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

density

1.077 g/mL at 25 °C

shipped in

ambient

storage temp.

2-8°C

Application

Used to create a density medium for the purification of lymphocytes and other mononuclear cells. Histopaque®-1077 is a solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077 +/- 0.001 g/ml. This medium facilitates rapid recovery of viable lymphocytes from small volumes of whole blood. This technique is suitable for use in cell mediated lympholysis and for human lymphocyte antigen (HLA)2 typing. It may also be employed as the initial isolation step prior to enumeration of T-, B-, and ‘null′ lymphocytes.

Legal Information

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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