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E7510

Sigma-Aldrich

Earle′s Balanced Salt Solution 10x

Without sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Earle’s Balanced Salts, EBSS

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: 10.0 g/L
NaHCO3: no
phenol red: 0.11 g/L

shipped in

ambient

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General description

Earle′s Balanced Salt Solution 10x is a saline solution with physiological pH. It is useful in inducing autophagy and promotes Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) maturation.

Application

Earle′s Balanced Salt Solution 10x has been used:
  • as an additive to the nutritive medium sheep nematode Trichostrongylus colubriformis eggs
  • in the swim-up method to prepare human sperm sample
  • in the steady-state medium to culture bovine primary mammary epithelial cells

Features and Benefits

  • Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance.
  • Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism.
  • When combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism.
  • Provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate at 1×.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Yanli Ren et al.
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Cell death & disease, 8(8), e3031-e3031 (2017-10-20)
Autophagy is a highly inducible system of intracellular degradation that occurs in lysosomes or vacuoles. Transmembrane 74 (TMEM74) has been shown to induce autophagy. However, the mechanism by which TMEM74 stimulates autophagy and the impacts of TMEM74-induced autophagy on tumor
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Autophagy is a housekeeping lysosomal degradation pathway important for cellular survival, homeostasis and function. Various disease models have shown that upregulation of autophagy may be beneficial to combat disease pathogenesis. However, despite several recently reported small-molecule screens for synthetic autophagy

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