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M9288

Sigma-Aldrich

Minimum Essential Medium Eagle

10 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
MEM
NACRES:
NA.75

Quality Level

400

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
HEPES: no
sodium pyruvate: no
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

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Minimum Essential Medium Eagle

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M0450

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sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

concentration

10 ×

concentration

-

concentration

-

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

Endotoxin, tested

components

NaHCO3: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
HEPES: no
sodium pyruvate: no
phenol red: yes

components

Earle’s salts (5% CO2): no
stable glutamine: no
Hanks’ salts (2% CO2): yes
phenol red: yes
NaHCO3: yes
sodium pyruvate: no
HEPES: no
glucose: no
L-glutamine: yes

components

phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes

components

glucose: 1.0 g/L
L-glutamine: 0.292 g/L
phenol red: 0.011 g/L
NaHCO3: 2.2 g/L

General description

Minimum Essential Medium (MEM), one of the most used synthetic cell culture media, is developed by Harry Eagle. It is used to cultivate various types of cells grown in monolayers.

Application

Minimum Essential Medium Eagle has been used as a transport media to maintain pig liver cells.

Reconstitution

Supplement with 0.292 g/L L-glutamine, 0.35 g/L sodium bicarbonate at 1×.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

Minimum Essential Medium Eagle (MEM) Specifications

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