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D1925

Sigma-Aldrich

Demecolcine solution

10 μg/mL in HBSS, ACF Qualified, BioXtra

Synonym(s):

Colcemid™ solution, N-Deacetyl-N-methylcolchicine solution

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About This Item

CAS Number:
477-30-5
MDL number:
MFCD00075459
UNSPSC Code:
12352207
PubChem Substance ID:
24893569
NACRES:
NA.75

Quality Level

400

sterility

sterile-filtered

product line

BioXtra

form

liquid

concentration

10 μg/mL in HBSS

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

SMILES string

CN[C@H]1CCc2cc(OC)c(OC)c(OC)c2C3=CC=C(OC)C(=O)C=C13

InChI

1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3/t15-/m0/s1

InChI key

NNJPGOLRFBJNIW-HNNXBMFYSA-N

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Application

Used for cell synchronization by arresting them at metaphase. Qualified for use in animal component free applications.

Legal Information

Colcemid is a trademark of Ciba-Geigy Corporation

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

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Dimitris Ioannou et al.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 17(4), 519-530 (2009-08-01)
In the field of nanotechnology, quantum dots (QDs) are a novel class of inorganic fluorochromes composed of nanometre-scale crystals made of a semiconductor material. Given the remarkable optical properties that they possess, they have been proposed as an ideal material
Ting-Yuan Tseng et al.
Molecules (Basel, Switzerland), 25(18) (2020-09-11)
Identification of the existence of G-quadruplex (G4) structure, from a specific G-rich sequence in cells, is critical to the studies of structural biology and drug development. Accumulating evidence supports the existence of G4 structure in vivo. Particularly, time-gated fluorescence lifetime
Vandana Relan et al.
PloS one, 8(3), e58132-e58132 (2013-03-22)
Malignant mesothelioma is an aggressive tumour of serosal surfaces most commonly pleura. Characterised cell lines represent a valuable tool to study the biology of mesothelioma. The aim of this study was to develop and biologically characterise six malignant mesothelioma cell
Mathew J Thayer
BioEssays : news and reviews in molecular, cellular and developmental biology, 34(9), 760-770 (2012-06-19)
Recent studies indicate that mammalian chromosomes contain discrete cis-acting loci that control replication timing, mitotic condensation, and stability of entire chromosomes. Disruption of the large non-coding RNA gene ASAR6 results in late replication, an under-condensed appearance during mitosis, and structural
Peter Burkovics et al.
Nucleic acids research, 44(7), 3176-3189 (2016-01-23)
Successful and accurate completion of the replication of damage-containing DNA requires mainly recombination and RAD18-dependent DNA damage tolerance pathways. RAD18 governs at least two distinct mechanisms: translesion synthesis (TLS) and template switching (TS)-dependent pathways. Whereas TS is mainly error-free, TLS
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