M-MSV-BALB/3T3

90030802, mouse embryo, Fibroblast

• UNSPSC Code: 41106514

Properties

• product name: M-MSV-BALB/3T3, 90030802
• biological source: mouse embryo
• growth mode: Adherent
• karyotype: Modal no. 57
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse BALB/3T3 embryo, Moloney sarcoma virus transformed

Cell Line Description

Derived from BALB/3T3 clone A31 (Sigma Catalogue number. 86110401) by infection with M-MSV. A M-MSV non-producer, but addition of MuLV permits rescue of the MSV genome. Cells produce tumours in suitable hosts; lack contact inhibition in vitro cultures; morphologically identical to virus releasing lines.

Application

Transformation and virus studies: M-MSV, MuLV

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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