J774A.1 Cell Line from mouse
91051511, mouse blood, Not specified
Synonym(s):
J774 A1 Cells, J774.A1 Cells, J774A1 Cells
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About This Item
UNSPSC Code:
41106514
product name
J774A.1 Cell Line from mouse, 91051511
biological source
mouse blood
description
Mouse BALB/c monocyte macrophage
growth mode
Semi-adherent
karyotype
Not specified
morphology
Not specified
products
Interleukin 1 (IL-1), lysozyme
receptors
Immunoglobulin, complement
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse BALB/c monocyte macrophage
Cell Line Description
Derived from a tumour in a female BALB/c mouse. Cell line produces IL-5 and lysozyme.
Application
J774A.1 cell line has been used to test the cytotoxicity of [1,2,3]triazolo[1,5-a]pyridinium salts (having leishmanicidal activity) from triazolopyridines. It has also been used to determine the antitrypanosomatid activity of flavonoid glycosides isolated from Delphinium sps.
Metabolic studies
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Cells are subcultured by harvesting attached and suspension cells. Remove majority of medium and centrifuge to harvest cells growing in suspension. Scrape adherent cells remaining in the flask. Resuspend cell pellet from centrifugation and combine with scraped cells. Seed into new flask at 1:3 to 1:4 split ratio i.e. seeding at 1-3 x 10,000 cells/cm2 Incubate at 37°C; 5% CO2. Please Note :- On receipt growing order cells may be in suspension. The cells and media should be removed from the flask and spun down to form a pellet. This should then be re-suspended in fresh media and a cell count performed. The cells should then be reseeded at 2-4 x 10E4/cm and should begin to show signs of re-attachment within 48 hours.
Other Notes
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Certificates of Analysis (COA)
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