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3T3 clone A31 Cell Line from mouse

86110401

Synonym(s):
3T3-A31 Cells, BALB/3T3 clone A31 Cells, BALB/c 3T3 clone A31 Cells

biological source

mouse embryo

description

Mouse BALB/c embryo

growth mode

Adherent

karyotype

2n = 40, hypotetraploid

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse BALB/c embryo

Cell Line Description

Cells have now lost their contact inhibition.

Application

3T3 clone A31 cell line from mouse has been used:
  • to study in vitro cytotoxicity and genotoxicity of retinyl palmitate–loaded poly(lactide-co-glycolide) nanoparticles for the topical treatment of skin diseases
  • for the characterization of the phototoxicity of Vemurafenib, which is a B-Raf serine/threonine kinase inhibitor
  • for the phototoxicity assessment of systemically applied compounds and drug candidates
Tumourigenicity and virus studies: SV40 and MSV

Culture Medium

DMEM + 2mM Glutamine + 5% New Born Calf Serum (NBCS) + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Certificate of Analysis

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Certificate of Origin

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