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recombinant
expressed in E. coli
Quality Level
form
liquid
packaging
vial of 50 μL
concentration
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
application(s)
CRISPR
selection
blasticidin
shipped in
dry ice
storage temp.
−20°C
Related Categories
General description
This product is a neomycin resistant lenti-Cas9 plasmid for generation of lentiviral particles. Use Sigma′s lentiviral Cas9 reagents to efficiently generate stable cell lines expressing Cas9 for CRISPR genome editing. Sigma′s lenti-Cas9 plasmid is one part of a two-vector CRISPR system with individual Cas9 and gRNA expression vectors. Please see the link above to order gRNA.
Application
Functional Genomics/Screening /Target Validation/Genome Editing
Features and Benefits
Use lentiviral particles to generate cells with stable expression of Cas9 nuclease.
Components
1ug of plasmid DNA, EF1a-Cas9-2A-Neomycin Lenti
Linkage
Click here to order gRNA plasmid and virus.
Storage Class
10 - Combustible liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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