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Key Documents

LVCAS9NEO

Sigma-Aldrich

Cas9 Neomycin Lenti Particles

Synonym(s):

Lenti Particles

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

packaging

vial of 200 μL

Quality Level

200

concentration

≥1 x 10 6 VP/ml virus (via p24 assay)

application(s)

CRISPR

selection

neomycin / G418

shipped in

dry ice

storage temp.

−70°C

General description

Ready-to-use Cas9 lentiviral particles are ideal for transducing a wide range of cell lines. The Cas9 Neomycin Lentiviral particles efficiently integrate Cas9 into the genomes of both dividing and non-dividing cell lines for use with CRISPR genome editing. Stably integrate Cas9 and Neo using the convenient, ready-to-use lentiviral particles while avoiding the tedious lentivirus production process.

Application

Functional Genomics/Screening /Target Validation/Genome Editing

Components

200 ul of purified lentiviral particles (minimum of 1 X 106 TU/ml via a p24 assay) expressing Cas9 only with blasticidin resistance.

Linkage

Click here to order gRNA plasmid and virus.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Sanger Whole Genome CRISPR Arrayed Libraries

Genome-wide loss-of-function screening is a powerful approach to discover genes and pathways that underlie biological processes. Now complete knockout is achievable with two optimized gRNAs per gene. Minimized clone number ensures the most specific screening possible while controlling time and cost.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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