A9325

L-Alanine 4-nitroanilide hydrochloride

protease substrate, ≥99% (TLC), powder

• Synonym(s): Ala-4-nitroanilide, H-Ala-pNA
• Empirical Formula (Hill Notation): C₉H₁₁N₃O₃ · HCl
• CAS Number: 31796-55-1
• Molecular Weight: 245.66
• Beilstein/REAXYS Number: 6303731
• EC Number: 250-811-0
• MDL number: MFCD00039088
• UNSPSC Code: 12352204
• eCl@ss: 32160406
• PubChem Substance ID: 24891436
• NACRES: NA.83

Properties

• product name: L-Alanine 4-nitroanilide hydrochloride, protease substrate
• Quality Level: 200
• assay: ≥99% (TLC)
• form: powder
• solubility: water: 50 mg/mL, clear to very slightly hazy
• storage temp.: −20°C
• SMILES string: Cl[H].C[C@H](N)C(=O)Nc1ccc(cc1)[N+]([O-])=O
• InChI: 1S/C9H11N3O3.ClH/c1-6(10)9(13)11-7-2-4-8(5-3-7)12(14)15;/h2-6H,10H2,1H3,(H,11,13);1H/t6-;/m0./s1
• InChI key: YEXRLSXNWLNHQR-RGMNGODLSA-N

Description

Application

L-Alanine 4-nitroanilide hydrochloride has been used to determine the activity of aminopeptidase in the intestinal segments and small intestine (jejunum and ileum).

Biochem/physiol Actions

L-Alanine-4-nitroanilide is used as a substrate to determine alanine aminopeptidase (AAP) activity. It is mainly chosen as a substrate, due to its relative specificity, higher hydrolysis rate and better solubility.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)