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A4418

Sigma-Aldrich

Ammonium sulfate

for molecular biology, ≥99.0%

Synonym(s):

Mascagnite

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About This Item

Linear Formula:
(NH4)2SO4
CAS Number:
7783-20-2
Molecular Weight:
132.14
EC Number:
231-984-1
MDL number:
MFCD00003391
UNSPSC Code:
12352314
eCl@ss:
38030408
PubChem Substance ID:
24890861
NACRES:
NA.31

grade

for molecular biology

Quality Level

200

assay

≥99.0%

form

powder

technique(s)

affinity binding assay: suitable
protein purification: suitable

impurities

≤0.005% Insolubles

pH

5.0-6 (25 °C, 132 g/L)

solubility

H2O: 0.667 g/mL

anion traces

chloride (Cl-): ≤5 ppm

cation traces

Cu: ≤5 ppm
Fe: ≤5 ppm
Zn: ≤5 ppm
heavy metals (as Pb): ≤2 ppm

absorption

≤0.025 at 280 at 1 M
≤0.03 at 260 at 1 M

suitability

suitable for flow cytometry
suitable for molecular biology

application(s)

metabolomics
microbiology

foreign activity

DNase, RNase, and protease, none detected

SMILES string

N.N.OS(O)(=O)=O

InChI

1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4)

InChI key

BFNBIHQBYMNNAN-UHFFFAOYSA-N

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General description

Ammonium sulfate is a versatile inorganic sulfate salt, plays crucial role in biochemical research, molecular biology, and cell culture. It serves as an indispensable tool for purifying antibodies and precipitating or fractionating proteins. Furthermore, it is instrumental in crystallographic analysis of nucleic acids and proteins and also used as a nitrogen supplement in synthetic laboratory media. Our premium-grade Ammonium Sulfate is meticulously crafted to be DNase, RNase, and Protease-free, ensuring its suitability for cutting-edge research.

Application

Ammonium sulfate helps in quick precipitation of celluar proteins. Ammonium sulfate may be used for the precipitation or fractionation of proteins or for purification of antibodies. It is also useful for crystallographic analysis of nucleic acids and proteins.Ammonium sulfate has been used
  • in the preparation of ′Blue Silver′ colloidal Coomassie stain for proteins.
  • to precipitate the Shiga-like toxin from the culture supernatant.
  • for precipitaion of viral particles from the culture supernatant.

Features and Benefits

  • Heavy metals (as Pb): ≤2 ppm
  • Free from DNase, RNase, and protease

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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William P Heal et al.
Nature protocols, 7(1), 105-117 (2011-12-24)
A protocol for selective and site-specific enzymatic labeling of proteins is described. The method exploits the protein co-/post-translational modification known as myristoylation, the transfer of myristic acid (a 14-carbon saturated fatty acid) to an N-terminal glycine catalyzed by the enzyme
Petia Kovatcheva-Datchary et al.
Cell reports, 26(13), 3772-3783 (2019-03-28)
The gut microbiota can modulate human metabolism through interactions with macronutrients. However, microbiota-diet-host interactions are difficult to study because bacteria interact in complex food webs in concert with the host, and many of the bacteria are not yet characterized. To
James F Hunter et al.
Environmental science & technology, 48(17), 10227-10234 (2014-08-06)
A large number of organic species emitted into the atmosphere contain cycloalkyl groups. While cyclic species are believed to be important secondary organic aerosol (SOA) precursors, the specific role of cyclic moieties (particularly for species with multiple or fused rings)
Kazunori Sugiura et al.
Biochemical and biophysical research communications, 457(3), 242-248 (2015-01-17)
Intracellular redox state is a critical factor for fundamental cellular functions, including regulation of the activities of various metabolic enzymes as well as ROS production and elimination. Genetically-encoded fluorescent redox sensors, such as roGFP (Hanson, G. T., et al. (2004))
Tse-Kai Fu et al.
Marine drugs, 17(6) (2019-06-19)
More than 80% of infectious bacteria form biofilm, which is a bacterial cell community surrounded by secreted polysaccharides, proteins and glycolipids. Such bacterial superstructure increases resistance to antimicrobials and host defenses. Thus, to control these biofilm-forming pathogenic bacteria requires antimicrobial
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