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IGEPAL® CA-630

Synonym(s):

Nonidet™ P 40, Nonylphenyl-polyethylenglycol, Octylphenoxy poly(ethyleneoxy)ethanol, branched

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About This Item

Linear Formula:
(C2H4O)nC14H22O
CAS Number:
9002-93-1
UNSPSC Code:
12161902
NACRES:
NA.25

description

non-ionic

Quality Level

100

form

liquid

mol wt

~603.0 g/mol

refractive index

n20/D 1.492

CMC

0.08 mM (20-25°C)

transition temp

cloud point 63-67  °C

density

1.06 g/mL at 20 °C

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤100 mg/kg

cation traces

Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤1000 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤1000 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

HLB

13

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChI key

JYCQQPHGFMYQCF-UHFFFAOYSA-N

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General description

IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes. It is chemically indistinguishable from Nonidet P-40.

Application

IGEPAL® CA-630 has been used:
  • In phosducin co-precipitation assay for pull down of Epitope-Tagged HAGγ1 and HAGγ1C71S
  • As a component of lysis buffer for cell culture and sample preparation for MAPK (mitogen-activated protein kinases) analysis, Immunoblotting
  • In the extraction of cells in Oil Red O staining procedure to visualise lipid formation in cells during adipogenesis
  • As a component of lysis buffer containing Tris-HCl, NaCl and EDTA for immunoblotting experiment

Other Notes

Nonionic, non-denaturing detergent

Quality

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Caution

May become turbid on storage; a clear product can be obtained on heating to 40°C.
May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

Legal Information

IGEPAL is a registered trademark of Solvay
Nonidet is a trademark of Shell Chemical Co.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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