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12138C

Sigma-Aldrich

Bovine Calf Serum

Iron-Fortified, USA origin, from formula-fed bovine calves, sterile-filtered, suitable for cell culture

Synonym(s):

Calf Serum, BCS, bovine calf sera, calf sera, calf serum

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About This Item

MDL number:
MFCD00243108
UNSPSC Code:
12352207
NACRES:
NA.71

biological source

bovine (calf) (formula-fed)

Quality Level

500

sterility

sterile-filtered

composition

hemoglobin, ≤35 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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Application

Iron-supplemented bovine serum as an alternative to fetal bovine serum in the CHO/HGPRT mutation assay, Oberly, et. al. Development of a more rapid, reduced serum culture system for Caco-2 monolayers and application to the biopharmaceutics classification system. Lentz et. al.

Testing Method

Endotoxin tested

Preparation Note

Each lot is supplemented to a constant iron concentration.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

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K A Lentz et al.
International journal of pharmaceutics, 200(1), 41-51 (2000-06-14)
The objectives were: (1) to develop a more rapid, reduced serum culture system for Caco-2 monolayers, relative to the traditional 21-day, 10% fetal bovine serum (FBS) system; and (2) to determine the biopharmaceutical drug classification of an oral therapeutic agent
T J Oberly et al.
Mutation research, 244(2), 105-109 (1990-06-01)
Iron-supplemented bovine calf serum (ICS) was found to be a viable alternative to fetal bovine serum (FBS) in the growth promotion and cloning efficiency of Chinese hamster ovary (CHO) cells that are used in the HGPRT mutation assay. Suspension cultures
D R Dahling et al.
Journal of virological methods, 27(3), 287-294 (1990-03-01)
Two studies comparing sewage-isolated and laboratory stock viruses were conducted to determine if alternative forms of serum or serum extenders could be used in place of fetal bovine serum without a significant loss of viral titer. In the first study
Wei Wang et al.
PloS one, 10(4), e0119005-e0119005 (2015-04-24)
Conjugated linoleic acid (CLA) reduces adiposity in human and mouse adipocytes. This outcome is achieved through a variety of biological responses including increased energy expenditure and fatty acid oxidation, increased inflammation, repression of fatty acid biosynthesis, attenuated glucose transport, and

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