T17917
Tricine
≥99% (titration), reagent grade, crystalline
Synonym(s):
N-[Tris(hydroxymethyl)methyl]glycine
About This Item
Recommended Products
biological source
synthetic
Quality Level
grade
reagent grade
assay
≥99% (titration)
form
crystalline
storage condition
(Tightly closed. Dry)
impurities
<0.5% water
color
white
useful pH range
7.4-8.8
pKa (25 °C)
8.1
mp
187 °C
solubility
water: 0.1 M, clear, colorless
cation traces
Pb: ≤5 ppm
storage temp.
room temp
SMILES string
OCC(CO)(CO)NCC(O)=O
InChI
1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
InChI key
SEQKRHFRPICQDD-UHFFFAOYSA-N
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General description
Tricine′s valuable properties for biological research include its zwitterionic nature, wide buffering range (pH 7.4-8.8), classification as a Good′s buffering agent, non-toxicity to cells, and high water solubility and clarity. These attributes make it suitable for metal-sensitive systems, stable pH maintenance, optimal biological applications, cell biology studies, and accurate measurements. Additionally, Tricine may find utilization in capillary zone electrophoresis, high-performance liquid chromatography, and ion exchange chromatography, demonstrating adaptability across analytical techniques.
Application
- Electrophoresis To separate low-weight peptides and proteins
- Crystallization of proteins of varying pH
- Resuspension of cell pellets
- Enzyme assays
- Tissue culture
Features and Benefits
- Ideal as a Biological buffer for Cell Biology and Biochemical research
- Effective Buffering from pH 7.4 - 8.8 with a pKa of 8.1 (25 °C)
Other Notes
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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