MilliporeSigma
All Photos(1)

T1165

Sigma-Aldrich

Tris-Tricine-SDS Buffer 10× Concentrate

NACRES:
NA.25

sterility

0.2 μm filtered

Quality Level

form

liquid

pH

8.05-8.30 (25 °C in water, high purity, 1:10)

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

Related Categories

General description

Tris-Tricine-SDS (TTS) running buffer is the cathode (upper reservoir) buffer for SDS−polyacrylamide gel electrophoresis of proteins using the Schagger and von Jagow method. The Schagger and von Jagow method is designed for the separation of small molecular weight proteins. It differs from the Laemmli method in that the glycine is replaced with tricine and the gel contains 1M Tris-HCl, pH 8.45 instead of 0.375 M Tris-HCl, pH 8.9. Dilution of the 10X TTS buffer produces a 1X cathode buffer containing 100 mM Tris, 100 mM tricine and 0.1% SDS. Recommended running conditions is 125 volts.

Application

Tris-Tricine-SDS Buffer 10× Concentrate is suitable for use as a running buffer for polyacrylamide based separation of bronchoalveolar lavage fluid (BALF).

Other Notes

1M Tris, 1M Tricine, 1% (w/v) SDS, pH approx. 8.2.

Analysis Note

Tested for use as a cathode buffer for Tris-Tricine gel electrophoresis to separate low molecular weight proteins.

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service