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D2935

Millipore

Decarboxylase broth base, Moeller

suitable for microbiology, NutriSelect® Plus

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

final pH

6.0±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
microbiology

suitability

nonselective and differential for Citrobacter spp.
nonselective and differential for Enterobacter spp.
nonselective and differential for Escherichia coli
nonselective and differential for Klebsiella spp.
nonselective and differential for Proteus spp.
nonselective and differential for Salmonella spp.
nonselective and differential for Serratia spp.
nonselective and differential for Shigella spp.
nonselective and differential for coliforms
nonselective and differential for enterobacteriaceae

Application

Used to differentiate bacteria on the basis of their ability to decarboxylate amino acids.

Components

Ingredients (g/L)
Peptic digest of animal tissue, 5.00
Beef extract, 5.00
Dextrose, 0.50
Bromocresol purple, 0.01
Cresol red, 0.005
Pyridoxal, 0.005

Preparation Note

Suspend 10.52 g of Decarboxylase Broth Base in 1000 ml of distilled water. Add 10 g of either L-Arginine (Cat. No. 11010), or L-Lysine (Cat. No. 62840), or L-Ornithine (Cat. No. 75470). Heat to dissolve the medium completely. When L-ornithine is added, readjust the pH. Dispense in 5 ml quantities in screwcapped tubes. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 10 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCF3309
BCCD0228
BCCB4457
BCBZ4527
BCBQ2131V

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