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SCT047

Sigma-Aldrich

BioTracker Cystine-FITC Live Cell Dye

Live cell imaging dye that measures cystine uptake at the single cell level used to detect glutathione (GSH) synthesis, reactive oxygen species (ROS) detoxification, T-cell activation and B-cell development.

Synonym(s):

Cellular cystine uptake detection, Live cell imaging probe

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About This Item

UNSPSC Code:
12352207

assay

≥98% (H-NMR)
≥98% (HPLC)
≥98% (LC/MS)
≥98% (elemental analysis)

technique(s)

cell based assay: suitable

detection method

fluorometric

shipped in

ambient

Related Categories

General description

Uptake of cystine allows accumulation of cysteine that is necessary for glutathione synthesis and reactive oxygen species (ROS) detoxification. After the uptake of cystine into the cytoplasm, the reducing intracellular environment leads to a separation of one cystine molecule into two cysteine molecules. A key function of cysteine is to maintain the cellular pools of glutathione (GSH). Cystine uptake is also required for T-cell activation and B-cell development.

The BioTracker Cystine-FITC dye is a live cell green fluorescent imaging probe that measures cystine uptake at the single cell level. The probe has been used to measure T-cell activation using traditional and imaging flow cytometry methods.

Spectral Properties
Absorbance: 450nm
Emission: 525nm

Application

Live cell fluorescent imaging
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

Quality

Purity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 625.65 g/mol

Physical form

Lyophilized

Storage and Stability

Store BioTracker Cystine-FITC Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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