MABN59

Anti-ACE2 Antibody, clone 4G5.1

clone 4G5.1, from mouse

• Synonym(s): Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• tag: GST tagged
• antibody form: purified antibody (protein G)
• antibody product type: primary antibodies
• clone: 4G5.1, monoclonal
• mol wt: ~62 kDa
• species reactivity: human
• technique(s): western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_068576
• UniProt accession no.: Q9BYF1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... ACE2(59272)

Description

General description

Angiotensin-converting enzyme 2 (ACE2) is a transmembrane protein belonging to the metallopeptidases (M2) family. It is a membrane-associated carboxypeptidase, expressed in the vascular endothelial cells of the heart, kidney, testis, gastrointestinal system and in the epithelial cells of lungs. ACE2 consists of an N-terminal peptidase domain (PD) and a C-terminal collectrin-like domain (CLD). It also comprises a single catalytic domain that is similar to that of ACE. The gene ACE2 is mapped to human chromosome location Xp22.2. ACE2 is a structural homolog ACE.

Specificity

This antibody recognizes ACE2.

Immunogen

GST-tagged recombinant protein corresponding to human ACE2.

Application

Anti-ACE2 Antibody, clone 4G5.1 detects level of ACE2 & has been validated for use in Western Blotting.

ELISA Analysis: A representative lot detected ACE2 in a direct ELISA.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Biochem/physiol Actions

Angiotensin-converting enzyme-2 (ACE2) produces angiotensin (1-9) by hydrolysis of angiotensin 1. Angiotensin (1-9) is a precursor for angiotensin (1-7). ACE2 may also directly produce angiotensin (1-7) by conversion of angiotensin II, which is involved in vasoconstriction.(2) Angiotensin (1-7) plays an important role in the renin-angiotensin system (RAS) signaling pathway and is involved in bradykinin-induced vasodilation. Hence, ACE2 antagonizes the effects of its homolog, ACE. ACE2 is insensitive to commonly used inhibitors such as lisinopril and captopril. This gene is upregulated in heart failure due to the overactivity of angiotensin II. ACE2 is studied for its potential therapeutic value for hypertension and heart failure. It binds to the viral envelope protein spike (S) protein and acts as a major entry receptor for severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2 in human cells. Hence, it is considered as a potential therapeutic target for COVID-19. ACE2 exhibits protection from lung injury.

Quality

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected ACE2 in 10 µg of HEK293 cell lysate.

Target description

~62 kDa observed. Uniport describes 2 isoforms at ~92 kDa (Isoform 1) and ~64 kDa (Isoform 2) due to alternative splicing.

Linkage

Replaces: MAB5676

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEK293 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable