Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

GE27-5325-01

Microspin™ G-25 Columns

Cytiva 27-5325-01

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41105501
NACRES:
NA.52

manufacturer/tradename

Cytiva 27-5325-01

packaging

pkg of 50 columns

particle size

20-80 μm (dry)

working range

2-13

General description

MicroSpin™ G-25 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.

MicroSpin™ G-25 columns can be used for any DNA greater than 10 bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or a labelling reaction. They will not remove or denature enzyme.

Features and Benefits

  • For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin-column chromatography.
  • Excellent for rapid purification of newly synthesized oligonucleotides >10-mers in 100 to 150 μl of deprotection solution using spin-column chromatography.
  • Convenient: Prepacked with Sephadex® G-25 DNA Grade and pre-equilibrated in distilled water containing 0.05% Kathon CG/ICP Biocide.
  • Ready to use: Requires less than 4 min from sample application to collection of purified product.
  • Tested in nickase, single and double-stranded exonuclease and RNase assays.
  • Can also be used for desalting/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 μl.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 20 to 25 °C. (Double distilled water containing 0.05% Kathon CG/ICP Biocide as preservative)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

MicroSpin is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

Storage Class

12 - Non Combustible Liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocols

DNA Cleanup using illustra™ GFX Kits

DNA cleanup using illustra™ GFX kits from Cytiva.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service