MABC544
Anti-PLK-4 Antibody, clone 6H5
clone 6H5, from mouse
Synonym(s):
Serine/threonine-protein kinase PLK4, Polo-like kinase 4, PLK-4, Serine/threonine-protein kinase 18, Serine/threonine-protein kinase Sak
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6H5, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG3κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PLK4(10733)
General description
Serine/threonine-protein kinase PLK4 (PLK-4) is a member of the protein kinase superfamily and plays an essential role in centriole duplication. PLK-4 triggers procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins. It may be involved in tumorigenesis since centrosome aberrations are frequently observed in tumors. PLK-4 may also be involved in trophoblast differentiation by phosphorylating HAND1, leading to the disruption between HAND1 and MDFIC and then activating HAND1.
Immunogen
Linear peptide corresponding to Human PLK-4.
Application
Detect Polo Like Kinase (PLK) using this mouse monoclonal antibody, Anti-PLK-4 Antibody, clone 6H5 validated for use in western blotting, ICC & IP.
Immunocytochemistry Analysis: A representative lot detected PLK-4 in U20S cell lysates transfected with control and Cep152 siRNA, but not U2OS cell lysates transfected with PLK4 siRNA. (Cizmecioglu, O. et al. (2010). J Cell Biol. 191(4):731-739).
Immunoprecipitation Analysis: A representative lot immunoprecipitated PLK-4 in HeLa cell lysates cotransfected with Flag-tagged Gcp6 and Myc-tagged Plk-4 (Bahtz, R. et al. (2012). J Cell Sci. 125 (Pt 2): 486-496).
Immunoprecipitation Analysis: A representative lot immunoprecipitated PLK-4 in HeLa cell lysates cotransfected with Flag-tagged Gcp6 and Myc-tagged Plk-4 (Bahtz, R. et al. (2012). J Cell Sci. 125 (Pt 2): 486-496).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Quality
Evaluated by Western Blotting in U2Os cell lysate.
Western Blotting Analysis: 4.0 µg/mL of this antibody detected PLK-4 in 10 µg of U2Os cell lysate.
Western Blotting Analysis: 4.0 µg/mL of this antibody detected PLK-4 in 10 µg of U2Os cell lysate.
Target description
109 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
U2Os cell lysate
U2Os cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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STIL binding to Polo-box 3 of PLK4 regulates centriole duplication.
Arquint, C; Gabryjonczyk, AM; Imseng, S; Bohm, R; Sauer, E; Hiller, S; Nigg, EA; Maier, T
eLife null
Massimo Bogliolo et al.
Genetics in medicine : official journal of the American College of Medical Genetics, 20(4), 458-463 (2017-08-25)
PurposeMutations in genes involved in Fanconi anemia (FA)/BRCA DNA repair pathway cause cancer susceptibility diseases including familial breast cancer and Fanconi anemia (FA). A single FA patient with biallelic FANCM mutations was reported in 2005 but concurrent FANCA pathogenic mutations
Midori Ohta et al.
Nature communications, 5, 5267-5267 (2014-10-25)
Formation of one procentriole next to each pre-existing centriole is essential for centrosome duplication, robust bipolar spindle assembly and maintenance of genome integrity. However, the mechanisms maintaining strict control over centriole copy number are incompletely understood. Here we show that
Onur Cizmecioglu et al.
The Journal of cell biology, 191(4), 731-739 (2010-11-10)
Both gain and loss of function studies have identified the Polo-like kinase Plk4/Sak as a crucial regulator of centriole biogenesis, but the mechanisms governing centrosome duplication are incompletely understood. In this study, we show that the pericentriolar material protein, Cep152
Satoko Yoshiba et al.
Journal of cell science, 132(12) (2019-06-06)
At the onset of procentriole formation, a structure called the cartwheel is formed adjacent to the pre-existing centriole. SAS-6 proteins are thought to constitute the hub of the cartwheel structure. However, the exact function of the cartwheel in the process
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