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386023

Sigma-Aldrich

Anti-Hsp47 Mouse mAb (M16.10A1)

liquid, clone M16.10A1, Calbiochem®

Synonym(s):

Anti-Colligin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

M16.10A1, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, porcine, chicken, canine, human, hamster, guinea pig, bovine, rat, monkey, sheep

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2b

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SERPINH1(871)

General description

Anti-Hsp47, mouse monoclonal, clone M16.10A1, recognizes the ~47 kDa Hsp47 protein in wide variety of species. It is validated for use in ELISA, EIA, WB, ICC, IF, IP, and IHC (paraffin sections).
Protein G purified mouse monoclonal antibody. Recognizes the ~47 kDa Hsp47 protein.
Recognizes the ~47 kDa Hsp47 protein.

Immunogen

Hsp47 from L6 cells
Rat

Application

ELISA (see comments)

Enzyme Immunoassay (see comments)

Immunoblotting (1 µg/ml, colorimetric)

Immunocytochemistry (1 µg/ml)

Immunofluorescence (5 µg/ml)

Immunoprecipitation (5 µg/ml)

Paraffin Sections (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 50% glycerol, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
3T3 cells, Human breast cancer tissue

Other Notes

Kuroda, K., et al. 1998. J. Invest. Dermatol.111, 1023.
Nandan, D., et al. 1998. Exp. Cell Res.179, 289.
Clark, E.P., et al. 1991. J. Biol. Chem.266, 17230.
Vaillancourt, J.P., and Cates, G.A. 1991. Biochem. J.274, 793.
Cates, G.A., et al. 1987. Biochem. Cell Biol. 65, 767.
Saga, S., et al. 1987. J. Cell Biol.105, 517.
Nagata, K., et al. 1986. J. Biol. Chem. 261, 7531.
This antibody has also been reported to work for ELISA and enzyme immunoassay. Variables associated with assay conditions will dictate the optimal dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yin Zhang et al.
Journal of oncology, 2021, 5570058-5570058 (2021-07-02)
In most cases, the carcinogenesis of colorectal cancer (CRC) follows the normal-adenoma-carcinoma (N-A-C) sequence. In this study, we aimed to identify the key proteins in the N-A-C sequence. Differentially expressed proteins (DEPs) in normal, adenoma, and carcinoma tissues were identified
Jie Dong et al.
Frontiers in pharmacology, 10, 1140-1140 (2019-10-22)
Exposure to insoluble particles in the lung elicits inflammatory responses that eliminate deposited particulates and repair damaged tissue. Overzealous or prolonged responses lead to chronic conditions, such as fibrosis and malignancy, which are frequently progressive and refractory to drug therapy

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