217356

Carboxypeptidase B, Porcine Pancreas

• Synonym(s): Carboxypeptidase B, Porcine Pancreas
• CAS Number: 9025-24-5
• Enzyme Commission number: 3.4.17.2 (BRENDA, IUBMB)
• MDL number: MFCD00081476
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• biological source: Porcine pancreas
• Quality Level: 100
• form: lyophilized
• specific activity: ≥100 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• pI: 6.0
• solubility: water: 1 mg/mL
• foreign activity: Carboxypeptidase A <1.0%; Chymotrypsin, none detected; Trypsin ≤5%
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
• InChI key: TWURVFFNODFJBJ-UHFFFAOYSA-N

Description

General description

Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides. Inhibited by EDTA and other Zn²⁺ chelators.

Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides. Inhibited by EDTA and other Zn²⁺ chelators. Has an optimal pH of 7.0-9.0 and a pI of 6.0.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Hip-Arg per min at 25°C, pH 7.7.

Physical form

Lyophilized from 360 µM HEPES, 1.41 µM zinc chloride, 1.41 mg/ml Trehalose, pH 7.4.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Folk, J.E. 1970. Methods Enzymol.19, 504.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable