Proteolytic hydrolysis of peptide bonds is recognized as an essential and ubiquitous mechanism for the regulation of a myriad of physiological processes. Four main classes of proteolytic enzymes have been routinely utilized to describe proteases. The serine proteases are probably the best characterized. This class of proteases includes trypsin, chymotrypsin and elastase. The cysteine protease class includes papain, calpain and lysosomal cathepsins. Aspartic proteases include pepsin and rennin. Metallo-proteases include thermolysin and carboxypeptidase A. Explore our comprehensive offering of rigorously tested proteinases for your protein workflow needs.
In order to assist with your protease selection, our Protease Finder allows researchers to locate endo- and exopeptidases that are required for precise protein cleavage. Facilitating the catalytic breakdown of proteins in smaller polypeptides or single amino acids, our proteases provide a comprehensive range of offerings to meet your protein research application needs.
Proteases are commonly used tools in proteomics research and suitable for digesting proteins into small peptide fragments for mass spectrometry analysis followed by sequencing (tandem MS). Trypsin generates peptides in the useful mass range for mass spectrometry and is recognized as the most used protease for protein identification. Explore our solution-stable recombinant SOLu-Trypsin products (EMS0004) (EMS0005) for additional information. Additionally, there are instances where separate or sequential digestion with other proteases may be a better choice for your protein of interest. We offer a variety of sequencing grade proteases that are appropriate for use in mass spectrometry sample preparation.
Proteases have commercial importance in different industrial and applied sectors and have multiple applications. Due to their wide variety of physiological characteristics, including hydrolysis at pH extremes or elevated temperatures, they ideal for use in the pharmaceutical, diagnostic, textile, food and beverage sectors. Specific applications for these proteases include but are not limited to; dietary fiber testing and clean-in-place additives for contaminate removal. We have a wide range of purified enzymes and enzyme mixtures for researching and developing new processes, products, and assays.
We offer two easy-to-use kits to detect trace amounts of protease, or to determine total protease activity, using the same methodologies utilized by our QC department for years. We have used these protocols to test thousands of samples for proteolytic activity.
The Protease Detection Kits are complete kits for detection of primary or trace protease activity by your choice of fluorometric or colorimetric detection. Everything you need, including control, standards, buffers, and substrate, is included. For convenience, the assay can be performed in either a cuvette or microplate format. Each kit contains enough reagents for 200 x 1 mL assays. For greater sensitivity when measuring low levels of protease activity, the Protease Fluorescent Detection kit (PF0100) is recommended. For more accurate quantification of primary protease activity, the Protease Colorimetric Detection kit is (PC0100) recommended.
Both the Fluorometric Detection Kit and the Colorimetric Detection Kit have been tested against representative samples of all four types of protease classes (serine, aspartic, cysteine and metalloproteases) to ensure broad application suitability.