Protease and phosphatase inhibitor cocktails are used to prevent proteolysis and dephosphorylation of proteins during extraction, sample preparation, and analysis. A variety of protease and phosphatase inhibitor cocktails as well as individual inhibitors are available to prevent sample degradation and preserve the native state of proteins during sample prep. Cocktails are designed and optimized for specific tissues including mammalian, plant, bacterial, and yeast.
During protein expression and isolation, endogenous proteases rapidly begin to degrade protein samples, reducing the quality and quantity of protein samples required for characterization and analysis. Protease inhibitor cocktails and highly specific protease inhibitors ensure protein integrity during protein extraction and preparation.
In cell signaling, target proteins are phosphorylated by protein kinases that transfer a phosphate group to specific sites. These phosphate groups are removed by endogenous protein phosphatases upon cell lysis. Phosphatase inhibitors help preserve the activity and state of phosphoproteins during cell lysis and protein extraction.
Roche offers a broad selection of convenient protease inhibitor cocktails as well as optimized lysis reagents to ensure maximum yields of intact and functional proteins.