QBD10193

Biotin-dPEG^®₃-NH₃⁺TFA^-

>95% (HPLC)

• Synonym(s): Biotin-PEG-Amine, Biotin-PEG₃-NH₃-TFA^-, Biotin-PEG₃-amine TFA salt, Biotin-PEG₃-ammonium trifluoroacetate
• Empirical Formula (Hill Notation): C₂₂H₃₉F₃N₄O₇S
• Molecular Weight: 560.63
• UNSPSC Code: 12352200
• NACRES: NA.22

Properties

• assay: >95% (HPLC)
• form: solid or viscous liquid
• reaction suitability: reaction type: Biotinylations; reaction type: Pegylations
• polymer architecture: shape: linear; functionality: monofunctional
• shipped in: ambient
• storage temp.: −20°C

Description

Features and Benefits

Biotin-dPEG₃-NH₃⁺TFA- (Biotin-dPEG₃-ammonium trifluoroacetate) reacts with activated esters (N-hydroxysuccinimide, 2,3,5,6-tetrafluorophenyl) of carboxylic acids under mildly basic conditions. It can be coupled directly to a carboxylic acid using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry. Also, the product can react with carbonyl groups (aldehydes or ketones) to form a Schiff base. The Schiff base can be reduced to a secondary amine using sodium cyanoborohydride if additional conjugate stability is necessary. Biotin-dPEG₃-NH₃⁺TFA- can be used to biotinylate antibodies useful in western blotting and immunohistochemical applications.

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Legal Information

Products Protected under U.S. Patent #s 7,888,536 & 8,637,711 and European Patent #s 1,594,440 & 2,750,681

dPEG is a registered trademark of Quanta BioDesign

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3