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LC/MS Analysis of Polar Amino Acids on Ascentis® Si (HILIC Mode)

LC/MS Analysis of Polar Amino Acids on Ascentis® Si (HILIC Mode) application for HPLC

Materiały

kolumna analityczna

Numer produktu
Opis
Cennik

Ascentis® Si HPLC Column

5 μm particle size, L × I.D. 10 cm × 2.1 mm

standardowe

Numer produktu
Opis
Cennik

L-Asparagine

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

L-Threonine

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

Serine

European Pharmacopoeia (EP) Reference Standard

Aspartic acid

United States Pharmacopeia (USP) Reference Standard

Glutamine

United States Pharmacopeia (USP) Reference Standard

Glutamic acid

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Si, 10 cm x 2.1 mm I.D., 5 μm particles (581508-U)

mobile phase

[A] 100 mM ammonium formate, pH 3.0 with formic acid; [B] water; [C] acetonitrile

gradient

5% A constant; 10 to 16% B, 85 to 79% C in 20 min

flow rate

0.3 mL/min

column temp.

35 °C

detector

ESI(+)

injection

2 μL

sample

0.1 g/L each in mobile phase

Opis

Informacje prawne

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany